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. 1991 Oct;44(10):824–825. doi: 10.1136/jcp.44.10.824

Beta-D-glucuronidase activity assay for rapid differentiation of species within beta-haemolytic group C and G streptococci.

N Cimolai 1, D Mah 1
PMCID: PMC496665  PMID: 1960215

Abstract

Methylumbelliferyl-conjugated enzyme substrates were assessed for their ability to differentiate beta haemolytic streptococci in Lancefield groups C and G. Both Streptococcus equisimilis (group C) and large colony human biotype group G strains were consistently differentiated from group C and G "Streptococcus milleri group" bacteria by their ability to hydrolyse the beta-D-glucuronide substrate. The test was completed in less than one hour.

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Selected References

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  1. Barnham M., Thornton T. J., Lange K. Nephritis caused by Streptococcus zooepidemicus (Lancefield group C). Lancet. 1983 Apr 30;1(8331):945–948. doi: 10.1016/s0140-6736(83)92078-0. [DOI] [PubMed] [Google Scholar]
  2. Bucher C., von Graevenitz A. Differentiation in throat cultures of group C and G streptococci from Streptococcus milleri with identical antigens. Eur J Clin Microbiol. 1984 Feb;3(1):44–45. doi: 10.1007/BF02032818. [DOI] [PubMed] [Google Scholar]
  3. Casemore D. P. Epidemiological aspects of human cryptosporidiosis. Epidemiol Infect. 1990 Feb;104(1):1–28. doi: 10.1017/s0950268800054480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cimolai N., Elford R. W., Bryan L., Anand C., Berger P. Do the beta-hemolytic non-group A streptococci cause pharyngitis? Rev Infect Dis. 1988 May-Jun;10(3):587–601. doi: 10.1093/clinids/10.3.587. [DOI] [PubMed] [Google Scholar]
  5. Clark R. B., Berrafati J. F., Janda J. M., Bottone E. J. Biotyping and exoenzyme profiling as an aid in the differentiation of human from bovine group G streptococci. J Clin Microbiol. 1984 Oct;20(4):706–710. doi: 10.1128/jcm.20.4.706-710.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duca E., Teodorovici G., Radu C., Vî A., Talaşman-Niculescu P., Bernescu E., Feldi C., Roşca V. A new nephritogenic streptococcus. J Hyg (Lond) 1969 Dec;67(4):691–698. doi: 10.1017/s0022172400042145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Efstratiou A. Outbreaks of human infection caused by pyogenic streptococci of Lancefield groups C and G. J Med Microbiol. 1989 Jul;29(3):207–219. doi: 10.1099/00222615-29-3-207. [DOI] [PubMed] [Google Scholar]
  8. Köhler W., Prokop O., Kühnemund O. Routine identification of group-C streptococci by means of an agglutinin (protectin) from the albumen gland of the edible snail, Helix pomatia. J Med Microbiol. 1973 Feb;6(1):127–130. doi: 10.1099/00222615-6-1-127. [DOI] [PubMed] [Google Scholar]
  9. Lawrence J., Yajko D. M., Hadley W. K. Incidence and characterization of beta-hemolytic Streptococcus milleri and differentiation from S. pyogenes (group A), S. equisimilis (group C), and large-colony group G streptococci. J Clin Microbiol. 1985 Nov;22(5):772–777. doi: 10.1128/jcm.22.5.772-777.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martin N. J., Kaplan E. L., Gerber M. A., Menegus M. A., Randolph M., Bell K., Cleary P. P. Comparison of epidemic and endemic group G streptococci by restriction enzyme analysis. J Clin Microbiol. 1990 Sep;28(9):1881–1886. doi: 10.1128/jcm.28.9.1881-1886.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ottensooser F., Nakamizo Y., Sato M., Miyamoto Y., Takizawa K. Lectins detecting group C streptococci. Infect Immun. 1974 May;9(5):971–973. doi: 10.1128/iai.9.5.971-973.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Slifkin M., Gil G. M. Rapid biochemical tests for the identification of groups A, B, C, F, and G streptococci from throat cultures. J Clin Microbiol. 1983 Jul;18(1):29–32. doi: 10.1128/jcm.18.1.29-32.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Whiley R. A., Hardie J. M. DNA-DNA hybridization studies and phenotypic characteristics of strains within the 'Streptococcus milleri group'. J Gen Microbiol. 1989 Oct;135(10):2623–2633. doi: 10.1099/00221287-135-10-2623. [DOI] [PubMed] [Google Scholar]

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