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. 2016 Feb 6;8(3):501–512. doi: 10.1080/19420862.2016.1145865

Figure 2.

Figure 2.

Mass spectra and location of protease cleavage sites during LOB12.3 and 3H3 Fab generation. (A) Intact mass spectra of reduced Fab generated by papain digestion of LOB12.3 IgG. The rat IgG1 hinge region sequence is shown above the spectra and sites of cleavage by papain (open triangles) or SpeB (filled triangle) are shown. (B) Intact mass spectra of reduced Fab generated by papain digestion of 3H3 IgG. The rat IgG2a hinge region sequence is shown above the spectra and sites of cleavage by papain are shown.