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. 1991 Sep;44(9):765–767. doi: 10.1136/jcp.44.9.765

Simple and specific test for measuring lipid peroxides in plasma.

P Görög 1, D C Kotak 1, I B Kovacs 1
PMCID: PMC496727  PMID: 1918407

Abstract

The specificity of an iodometric assay for measuring lipid peroxides in lipoproteins was tested, compared with the fluorimetric thiobarbituric acid assay, and adopted for detecting lipid peroxide in plasma samples. Oxidation of low density lipoproteins in vitro by Cu2+, lipoxidase, and phagocytosing polymorphonuclear leucocytes was sensitively detected by the iodometric assay. Unlike the thiobarbituric acid assay, neither non-lipid substances commonly present in plasma, nor platelet or polymorphonuclear leucocyte by-products interfered with the iodometric assay. The iodometric assay measured a normal mean (SD) plasma lipid peroxide concentration of 10.8 (2.1) microM; n = 63. Two weeks after the start of a high cholesterol diet in rabbits (n = 5), a sixfold increase in plasma lipid peroxide concentrations was measured by iodometric assay. The specificity of a simple and sensitive iodometric test of lipid peroxidation was superior to that of the thiobarbituric acid assay. This iodometric assay should therefore provide a much more accurate assessment of lipid peroxide in plasma samples.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviram M., Dankner G., Brook J. G. Platelet secretory products increase low density lipoprotein oxidation, enhance its uptake by macrophages, and reduce its fluidity. Arteriosclerosis. 1990 Jul-Aug;10(4):559–563. doi: 10.1161/01.atv.10.4.559. [DOI] [PubMed] [Google Scholar]
  2. Davies S. W., Ranjadayalan K., Wickens D. G., Dormandy T. L., Timmis A. D. Lipid peroxidation associated with successful thrombolysis. Lancet. 1990 Mar 31;335(8692):741–743. doi: 10.1016/0140-6736(90)90866-4. [DOI] [PubMed] [Google Scholar]
  3. Fitzgerald D. J., Catella F., Roy L., FitzGerald G. A. Marked platelet activation in vivo after intravenous streptokinase in patients with acute myocardial infarction. Circulation. 1988 Jan;77(1):142–150. doi: 10.1161/01.cir.77.1.142. [DOI] [PubMed] [Google Scholar]
  4. Gutteridge J. M., Halliwell B. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem Sci. 1990 Apr;15(4):129–135. doi: 10.1016/0968-0004(90)90206-q. [DOI] [PubMed] [Google Scholar]
  5. Görög P. Activation of human blood monocytes by oxidized polyunsaturated fatty acids: a possible mechanism for the generation of lipid peroxides in the circulation. Int J Exp Pathol. 1991 Apr;72(2):227–237. [PMC free article] [PubMed] [Google Scholar]
  6. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Janero D. R., Burghardt B. Thiobarbituric acid-reactive malondialdehyde formation during superoxide-dependent, iron-catalyzed lipid peroxidation: influence of peroxidation conditions. Lipids. 1989 Feb;24(2):125–131. doi: 10.1007/BF02535249. [DOI] [PubMed] [Google Scholar]
  8. Kovacs I. B., Meyrick Thomas R. H., Mackay A. R., Rustin M. H., Kirby J. D. Increased chemiluminescence of polymorphonuclear leucocytes from patients with progressive systemic sclerosis. Clin Sci (Lond) 1986 Mar;70(3):257–261. doi: 10.1042/cs0700257. [DOI] [PubMed] [Google Scholar]
  9. Ohkawa H., Ohishi N., Yagi K. Reaction of linoleic acid hydroperoxide with thiobarbituric acid. J Lipid Res. 1978 Nov;19(8):1053–1057. [PubMed] [Google Scholar]
  10. Steinberg D., Parthasarathy S., Carew T. E., Khoo J. C., Witztum J. L. Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med. 1989 Apr 6;320(14):915–924. doi: 10.1056/NEJM198904063201407. [DOI] [PubMed] [Google Scholar]
  11. Stringer M. D., Görög P. G., Freeman A., Kakkar V. V. Lipid peroxides and atherosclerosis. BMJ. 1989 Feb 4;298(6669):281–284. doi: 10.1136/bmj.298.6669.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wade C. R., van Rij A. M. Plasma malondialdehyde, lipid peroxides, and the thiobarbituric acid reaction. Clin Chem. 1989 Feb;35(2):336–336. [PubMed] [Google Scholar]
  13. Yamamoto Y., Brodsky M. H., Baker J. C., Ames B. N. Detection and characterization of lipid hydroperoxides at picomole levels by high-performance liquid chromatography. Anal Biochem. 1987 Jan;160(1):7–13. doi: 10.1016/0003-2697(87)90606-3. [DOI] [PubMed] [Google Scholar]
  14. el-Saadani M., Esterbauer H., el-Sayed M., Goher M., Nassar A. Y., Jürgens G. A spectrophotometric assay for lipid peroxides in serum lipoproteins using a commercially available reagent. J Lipid Res. 1989 Apr;30(4):627–630. [PubMed] [Google Scholar]

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