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. 1991 Sep;44(9):768–771. doi: 10.1136/jcp.44.9.768

Immune responses to Helicobacter pylori in children with recurrent abdominal pain.

J E Crabtree 1, M J Mahony 1, J D Taylor 1, R V Heatley 1, J M Littlewood 1, D S Tompkins 1
PMCID: PMC496728  PMID: 1918408

Abstract

The systemic immune response to Helicobacter pylori was examined in 69 children with recurrent abdominal pain and upper gastrointestinal symptoms. Twenty one (30%) children were histologically positive for H pylori. Eighteen of the 21 positive subjects and two H pylori negative subjects (one with normal mucosa, one with lymphocytic gastritis) were positive for H pylori IgG antibodies by enzyme linked immunosorbent assay (ELISA) (86% sensitivity, 98% specificity). In children with H pylori associated gastritis, there was a significant positive correlation (p less than 0.05) between IgG antibody titres and patient age. Intra-assay comparison of sera from histologically negative adults with those of histologically negative children showed that the cut off for positivity in the ELISA for adults was greater than that for children. Immunoblotting showed IgG positivity in 20 of the 21 patients with H pylori infection (95% sensitivity). Both ELISA and immunoblotting for IgA and IgM H pylori antibodies had poor discriminatory value for determining infection. Serological detection of H pylori IgG antibodies seems to be valuable in the assessment of children presenting with recurrent abdominal pain and other gastrointestinal symptoms, but assays must first be validated in paediatric populations.

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Selected References

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  1. Apel I., Jacobs E., Kist M., Bredt W. Antibody response of patients against a 120 kDa surface protein of Campylobacter pylori. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Apr;268(2):271–276. doi: 10.1016/s0176-6724(88)80012-9. [DOI] [PubMed] [Google Scholar]
  2. Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984 Jan;136(1):175–179. doi: 10.1016/0003-2697(84)90320-8. [DOI] [PubMed] [Google Scholar]
  3. Cadranel S., Goossens H., De Boeck M., Malengreau A., Rodesch P., Butzler J. P. Campylobacter pyloridis in children. Lancet. 1986 Mar 29;1(8483):735–736. doi: 10.1016/s0140-6736(86)91120-7. [DOI] [PubMed] [Google Scholar]
  4. Crabtree J. E., Shallcross T. M., Heatley R. V., Wyatt J. I. Evaluation of a commercial ELISA for serodiagnosis of Helicobacter pylori infection. J Clin Pathol. 1991 Apr;44(4):326–328. doi: 10.1136/jcp.44.4.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Czinn S., Carr H., Sheffler L., Aronoff S. Serum IgG antibody to the outer membrane proteins of Campylobacter pylori in children with gastroduodenal disease. J Infect Dis. 1989 Mar;159(3):586–589. doi: 10.1093/infdis/159.3.586. [DOI] [PubMed] [Google Scholar]
  6. Dent J. C., McNulty C. A., Uff J. S., Gear M. W., Wilkinson S. P. Campylobacter pylori urease: a new serological test. Lancet. 1988 Apr 30;1(8592):1002–1002. doi: 10.1016/s0140-6736(88)91827-2. [DOI] [PubMed] [Google Scholar]
  7. Dixon M. F., Wyatt J. I., Burke D. A., Rathbone B. J. Lymphocytic gastritis--relationship to Campylobacter pylori infection. J Pathol. 1988 Feb;154(2):125–132. doi: 10.1002/path.1711540204. [DOI] [PubMed] [Google Scholar]
  8. Drumm B., O'Brien A., Cutz E., Sherman P. Campylobacter pyloridis-associated primary gastritis in children. Pediatrics. 1987 Aug;80(2):192–195. [PubMed] [Google Scholar]
  9. Drumm B., Perez-Perez G. I., Blaser M. J., Sherman P. M. Intrafamilial clustering of Helicobacter pylori infection. N Engl J Med. 1990 Feb 8;322(6):359–363. doi: 10.1056/NEJM199002083220603. [DOI] [PubMed] [Google Scholar]
  10. Evans D. J., Jr, Evans D. G., Graham D. Y., Klein P. D. A sensitive and specific serologic test for detection of Campylobacter pylori infection. Gastroenterology. 1989 Apr;96(4):1004–1008. doi: 10.1016/0016-5085(89)91616-8. [DOI] [PubMed] [Google Scholar]
  11. Glassman M. S., Dallal S., Berezin S. H., Bostwick H. E., Newman L. J., Perez-Perez G. I., Blaser M. J. Helicobacter pylori-related gastroduodenal disease in children. Diagnostic utility of enzyme-linked immunosorbent assay. Dig Dis Sci. 1990 Aug;35(8):993–997. doi: 10.1007/BF01537248. [DOI] [PubMed] [Google Scholar]
  12. Glassman M. S., Schwarz S. M., Medow M. S., Beneck D., Halata M., Berezin S., Newman L. J. Campylobacter pylori-related gastrointestinal disease in children. Incidence and clinical findings. Dig Dis Sci. 1989 Oct;34(10):1501–1504. doi: 10.1007/BF01537100. [DOI] [PubMed] [Google Scholar]
  13. Goodwin C. S., Blincow E., Peterson G., Sanderson C., Cheng W., Marshall B., Warren J. R., McCulloch R. Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa. J Infect Dis. 1987 Mar;155(3):488–494. doi: 10.1093/infdis/155.3.488. [DOI] [PubMed] [Google Scholar]
  14. Investigating young patients with dyspepsia. Lancet. 1989 Jan 7;1(8628):50–51. [PubMed] [Google Scholar]
  15. Kyhse-Andersen J. Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods. 1984 Dec;10(3-4):203–209. doi: 10.1016/0165-022x(84)90040-x. [DOI] [PubMed] [Google Scholar]
  16. Mahony M. J., Wyatt J. I., Littlewood J. M. Campylobacter pylori gastritis. Arch Dis Child. 1988 Jun;63(6):654–655. doi: 10.1136/adc.63.6.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Marshall B. J., Warren J. R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984 Jun 16;1(8390):1311–1315. doi: 10.1016/s0140-6736(84)91816-6. [DOI] [PubMed] [Google Scholar]
  18. Mégraud F., Brassens-Rabbé M. P., Denis F., Belbouri A., Hoa D. Q. Seroepidemiology of Campylobacter pylori infection in various populations. J Clin Microbiol. 1989 Aug;27(8):1870–1873. doi: 10.1128/jcm.27.8.1870-1873.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oderda G., Vaira D., Holton J., Dowsett J. F., Ansaldi N. Serum pepsinogen I and IgG antibody to Campylobacter pylori in non-specific abdominal pain in childhood. Gut. 1989 Jul;30(7):912–916. doi: 10.1136/gut.30.7.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perez-Perez G. I., Dworkin B. M., Chodos J. E., Blaser M. J. Campylobacter pylori antibodies in humans. Ann Intern Med. 1988 Jul 1;109(1):11–17. doi: 10.7326/0003-4819-109-1-11. [DOI] [PubMed] [Google Scholar]
  21. Plebani A., Ugazio A. G., Avanzini M. A., Massimi P., Zonta L., Monafo V., Burgio G. R. Serum IgG subclass concentrations in healthy subjects at different age: age normal percentile charts. Eur J Pediatr. 1989 Dec;149(3):164–167. doi: 10.1007/BF01958271. [DOI] [PubMed] [Google Scholar]
  22. Rathbone B. J., Wyatt J. I., Worsley B. W., Shires S. E., Trejdosiewicz L. K., Heatley R. V., Losowsky M. S. Systemic and local antibody responses to gastric Campylobacter pyloridis in non-ulcer dyspepsia. Gut. 1986 Jun;27(6):642–647. doi: 10.1136/gut.27.6.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Whitehead R., Truelove S. C., Gear M. W. The histological diagnosis of chronic gastritis in fibreoptic gastroscope biopsy specimens. J Clin Pathol. 1972 Jan;25(1):1–11. doi: 10.1136/jcp.25.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yeung C. K., Fu K. H., Yuen K. Y., Ng W. F., Tsang T. M., Branicki F. J., Saing H. Helicobacter pylori and associated duodenal ulcer. Arch Dis Child. 1990 Nov;65(11):1212–1216. doi: 10.1136/adc.65.11.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. von Wulffen H., Grote H. J., Gatermann S., Löning T., Berger B., Buhl C. Immunoblot analysis of immune response to Campylobacter pylori and its clinical associations. J Clin Pathol. 1988 Jun;41(6):653–659. doi: 10.1136/jcp.41.6.653. [DOI] [PMC free article] [PubMed] [Google Scholar]

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