Abstract
The distribution of two major basement membrane components, type IV collagen and laminin, was studied within the perisinusoidal space of Disse in normal human liver using (i) an immunoperoxidase method for light microscopy and (ii) immunogold labelling for ultrastructural localisation. Although immunoreactivity depended on the mode of tissue fixation, both proteins could be identified at this site using a panel of affinity purified antibodies. These findings indicate that these proteins are normal constituents of the perisinusoidal extracellular matrix, and refute the hypothesis that capillarization of the sinusoids in chronic liver disease results from neo-expression of laminin in the space of Disse.
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- Becker J., Schuppan D., Hahn E. G., Albert G., Reichart P. The immunohistochemical distribution of collagens type IV, V, VI and of laminin in the human oral mucosa. Arch Oral Biol. 1986;31(3):179–186. doi: 10.1016/0003-9969(86)90125-1. [DOI] [PubMed] [Google Scholar]
- Bianchi F. B., Biagini G., Ballardini G., Cenacchi G., Faccani A., Pisi E., Laschi R., Liotta L., Garbisa S. Basement membrane production by hepatocytes in chronic liver disease. Hepatology. 1984 Nov-Dec;4(6):1167–1172. doi: 10.1002/hep.1840040612. [DOI] [PubMed] [Google Scholar]
- Burt A. D., Griffiths M. R., Schuppan D., Voss B., MacSween R. N. Ultrastructural localization of extracellular matrix proteins in liver biopsies using ultracryomicrotomy and immuno-gold labelling. Histopathology. 1990 Jan;16(1):53–58. doi: 10.1111/j.1365-2559.1990.tb01060.x. [DOI] [PubMed] [Google Scholar]
- Clément B., Rescan P. Y., Baffet G., Loréal O., Lehry D., Campion J. P., Guillouzo A. Hepatocytes may produce laminin in fibrotic liver and in primary culture. Hepatology. 1988 Jul-Aug;8(4):794–803. doi: 10.1002/hep.1840080417. [DOI] [PubMed] [Google Scholar]
- Folkvord J. M., Viders D., Coleman-Smith A., Clark R. A. Optimization of immunohistochemical techniques to detect extracellular matrix proteins in fixed skin specimens. J Histochem Cytochem. 1989 Jan;37(1):105–113. doi: 10.1177/37.1.2461979. [DOI] [PubMed] [Google Scholar]
- Hahn E., Wick G., Pencev D., Timpl R. Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin, and fibronectin. Gut. 1980 Jan;21(1):63–71. doi: 10.1136/gut.21.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maher J. J., Friedman S. L., Roll F. J., Bissell D. M. Immunolocalization of laminin in normal rat liver and biosynthesis of laminin by hepatic lipocytes in primary culture. Gastroenterology. 1988 Apr;94(4):1053–1062. doi: 10.1016/0016-5085(88)90566-5. [DOI] [PubMed] [Google Scholar]
- Wisse E., De Zanger R. B., Charels K., Van Der Smissen P., McCuskey R. S. The liver sieve: considerations concerning the structure and function of endothelial fenestrae, the sinusoidal wall and the space of Disse. Hepatology. 1985 Jul-Aug;5(4):683–692. doi: 10.1002/hep.1840050427. [DOI] [PubMed] [Google Scholar]