Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1991 Aug;44(8):699. doi: 10.1136/jcp.44.8.699

Acanthamoeba keratitis: experience in a non-specialist microbiology laboratory.

D Anderson 1, S S Soo 1, H Towler 1
PMCID: PMC496773  PMID: 1890209

Abstract

Acanthamoeba keratitis is an unusual complication of wearing soft contact lenses: a case was diagnosed by culturing corneal scrapings. Acanthamoeba are tolerant of variations in osmolarity in the culture media. Page's saline, recommended for culture agar plates, can easily be substituted with salines which are suitable for cell culture media, thereby avoiding the cumbersome manufacture of special saline. Early diagnosis as a result of successful isolation may avoid the need for keratoplasty.

Full text

PDF

Page 699

699

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen E. J., Buchanan H. W., Laughrea P. A., Adams C. P., Galentine P. G., Visvesvara G. S., Folberg R., Arentsen J. J., Laibson P. R. Diagnosis and management of Acanthamoeba keratitis. Am J Ophthalmol. 1985 Sep 15;100(3):389–395. doi: 10.1016/0002-9394(85)90499-4. [DOI] [PubMed] [Google Scholar]
  2. JAHNES W. G., FULLMER H. M. Free living amoebae as contaminants in monkey kidney tissue culture. Proc Soc Exp Biol Med. 1957 Nov;96(2):484–488. doi: 10.3181/00379727-96-23515. [DOI] [PubMed] [Google Scholar]
  3. Ma P., Visvesvara G. S., Martinez A. J., Theodore F. H., Daggett P. M., Sawyer T. K. Naegleria and Acanthamoeba infections: review. Rev Infect Dis. 1990 May-Jun;12(3):490–513. doi: 10.1093/clinids/12.3.490. [DOI] [PubMed] [Google Scholar]
  4. Naginton J., Watson P. G., Playfair T. J., McGill J., Jones B. R., Steele A. D. Amoebic infection of the eye. Lancet. 1974 Dec 28;2(7896):1537–1540. doi: 10.1016/s0140-6736(74)90285-2. [DOI] [PubMed] [Google Scholar]
  5. Wright P., Warhurst D., Jones B. R. Acanthamoeba keratitis successfully treated medically. Br J Ophthalmol. 1985 Oct;69(10):778–782. doi: 10.1136/bjo.69.10.778. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES