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. 1991 Jul;44(7):549–553. doi: 10.1136/jcp.44.7.549

Expression of intrahepatic hepatitis D viral antigen in chronic hepatitis D virus infection.

J Y Lau 1, L J Hansen 1, V G Bain 1, K Chaggar 1, H M Smith 1, B C Portman 1, D Vergani 1, G J Alexander 1, R Williams 1
PMCID: PMC496792  PMID: 1856285

Abstract

To elucidate the biological importance of intrahepatic hepatitis D virus antigen, its expression was correlated with biochemical and histological inflammatory activity in 98 biopsy specimens from 68 patients seropositive for total antibody to the virus. Seventy five specimens were positive for intrahepatic nuclear antigen for HDV antigen accompanied by cytoplasmic HDV antigen in only one biopsy specimen. This group had significantly higher serum transaminase activities and inflammatory activity than the remaining cases that were negative for HDV antigen. Among the group positive for HDV antigen, there was no correlation between the proportion of hepatocytes containing HDV antigen and either serum transaminase activity or histological inflammatory indices. In 22 HDV antigen positive patients who had follow up biopsy specimens taken at a median of two years, the proportion with cirrhosis increased from 36% to 73%. Serum transaminase activities remained the same during this period, but the proportion of HDV antigen positive cells dropped. Follow up of 51 patients showed that 21 died or underwent liver transplantation within three years. The absence of an association between intrahepatic HDV antigen expression and progression of histological liver damage does not support the view that HDV is directly cytopathic to hepatocytes. Immune mediated mechanisms may have a role in the pathogenesis of chronic liver disease related to HDV infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chu C. M., Liaw Y. F. Studies on the composition of the mononuclear cell infiltrates in liver from patients with chronic active delta hepatitis. Hepatology. 1989 Dec;10(6):911–915. doi: 10.1002/hep.1840100603. [DOI] [PubMed] [Google Scholar]
  2. Fagan E. A., Guarner P., Perera S. D., Trowbridge R., Rolando N., Davison F., Williams R. Quantitation of hepatitis B virus DNA (HBV DNA) in serum using the spot hybridization technique and scintillation counting. J Virol Methods. 1985 Dec;12(3-4):251–262. doi: 10.1016/0166-0934(85)90136-3. [DOI] [PubMed] [Google Scholar]
  3. Govindarajan S., Gupta S., Valinluck B., Redeker A. G. Correlation of IgM anti-hepatitis D virus (HDV) to HDV RNA in sera of chronic HDV. Hepatology. 1989 Jul;10(1):34–35. doi: 10.1002/hep.1840100108. [DOI] [PubMed] [Google Scholar]
  4. Kojima T., Callea F., Desmyter J., Desmet V. J. Immune electron microscopy of hepatitis delta-antigen in hepatocytes. Lab Invest. 1986 Aug;55(2):217–225. [PubMed] [Google Scholar]
  5. Krogsgaard K., Aldershvile J., Kryger P., Andersson P., Nielsen J. O., Hansson B. G. Hepatitis B virus DNA, HBeAg and delta infection during the course from acute to chronic hepatitis B virus infection. Hepatology. 1985 Sep-Oct;5(5):778–782. doi: 10.1002/hep.1840050512. [DOI] [PubMed] [Google Scholar]
  6. Krogsgaard K., Kryger P., Aldershvile J., Andersson P., Sørensen T. I., Nielsen J. O. Delta-infection and suppression of hepatitis B virus replication in chronic HBsAg carriers. Hepatology. 1987 Jan-Feb;7(1):42–45. doi: 10.1002/hep.1840070110. [DOI] [PubMed] [Google Scholar]
  7. Madejón A., Castillo I., Bartolomé J., Melero M., Campillo M. L., Porres J. C., Moreno A., Carreño V. Detection of HDV-RNA by PCR in serum of patients with chronic HDV infection. J Hepatol. 1990 Nov;11(3):381–384. doi: 10.1016/0168-8278(90)90225-g. [DOI] [PubMed] [Google Scholar]
  8. Negro F., Baldi M., Bonino F., Rocca G., Demartini A., Passarino G., Maran E., Lavarini C., Rizzetto M., Verme G. Chronic HDV (hepatitis delta virus) hepatitis. Intrahepatic expression of delta antigen, histologic activity and outcome of liver disease. J Hepatol. 1988 Feb;6(1):8–14. doi: 10.1016/s0168-8278(88)80457-4. [DOI] [PubMed] [Google Scholar]
  9. Negro F., Bonino F., Di Bisceglie A., Hoofnagle J. H., Gerin J. L. Intrahepatic markers of hepatitis delta virus infection: a study by in situ hybridization. Hepatology. 1989 Dec;10(6):916–920. doi: 10.1002/hep.1840100604. [DOI] [PubMed] [Google Scholar]
  10. Rizzetto M., Canese M. G., Gerin J. L., London W. T., Sly D. L., Purcell R. H. Transmission of the hepatitis B virus-associated delta antigen to chimpanzees. J Infect Dis. 1980 May;141(5):590–602. doi: 10.1093/infdis/141.5.590. [DOI] [PubMed] [Google Scholar]
  11. Rizzetto M., Verme G. Delta hepatitis--present status. J Hepatol. 1985;1(2):187–193. doi: 10.1016/s0168-8278(85)80766-2. [DOI] [PubMed] [Google Scholar]
  12. Smedile A., Rizzetto M., Denniston K., Bonino F., Wells F., Verme G., Consolo F., Hoyer B., Purcell R. H., Gerin J. L. Type D hepatitis: the clinical significance of hepatitis D virus RNA in serum as detected by a hybridization-based assay. Hepatology. 1986 Nov-Dec;6(6):1297–1302. doi: 10.1002/hep.1840060612. [DOI] [PubMed] [Google Scholar]
  13. Trevisan A., Gudat F., Busachi C., Stöcklin E., Bianchi L. An improved method for HBcAg demonstration in paraffin-embedded liver tissue. Liver. 1982 Dec;2(4):331–339. doi: 10.1111/j.1600-0676.1982.tb00831.x. [DOI] [PubMed] [Google Scholar]
  14. Verme G., Amoroso P., Lettieri G., Pierri P., David E., Sessa F., Rizzi R., Bonino F., Recchia S., Rizzetto M. A histological study of hepatitis delta virus liver disease. Hepatology. 1986 Nov-Dec;6(6):1303–1307. doi: 10.1002/hep.1840060613. [DOI] [PubMed] [Google Scholar]

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