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. 1991 May;44(5):406–409. doi: 10.1136/jcp.44.5.406

In situ evidence for HPV 16, 18, 33 integration in cervical squamous cell cancer in Britain and South Africa.

K Cooper 1, C S Herrington 1, A K Graham 1, M F Evans 1, J O McGee 1
PMCID: PMC496873  PMID: 1646238

Abstract

In a previous study three types of HPV signal were described in CIN. It was suggested that a type 1 signal represented episomal HPV while a type 2 signal represented integrated HPV; and a type 3 signal was indicative of both episomal and integrated HPV. To test this hypothesis 91 squamous cell cancers (SCC) of the cervix from Britain and South Africa were examined for HPV 6, 11, 16, 18, 31, 33, 35. Of the South African group (n = 69) 64% contained HPV types 16 (n = 29) and 18 (n = 15). The SCC in the British group (n = 22) contained HPV 16 and HPV 33 in 12 and three cases, respectively. Of the HPV positive biopsy specimens, 86% showed a type 2 signal in keratinising and non-keratinising tumours and the remainder a type 3 signal. Type 3 signal was present only in keratinising tumours. The presence of punctate signal in 100% of HPV containing SCC, together with localisation of HPV signal to sister chromatids in tumour cell mitotic figures in vivo, provides further evidence for type 2, and the punctate component of type 3 signal representing viral integration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boshart M., Gissmann L., Ikenberg H., Kleinheinz A., Scheurlen W., zur Hausen H. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 1984 May;3(5):1151–1157. doi: 10.1002/j.1460-2075.1984.tb01944.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chang F. Role of papillomaviruses. J Clin Pathol. 1990 Apr;43(4):269–276. doi: 10.1136/jcp.43.4.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper K., Herrington C. S., Graham A. K., Evans M. F., McGee J. O. In situ human papillomavirus (HPV) genotyping of cervical intraepithelial neoplasia in South African and British patients: evidence for putative HPV integration in vivo. J Clin Pathol. 1991 May;44(5):400–405. doi: 10.1136/jcp.44.5.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dürst M., Gissmann L., Ikenberg H., zur Hausen H. A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3812–3815. doi: 10.1073/pnas.80.12.3812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dürst M., Kleinheinz A., Hotz M., Gissmann L. The physical state of human papillomavirus type 16 DNA in benign and malignant genital tumours. J Gen Virol. 1985 Jul;66(Pt 7):1515–1522. doi: 10.1099/0022-1317-66-7-1515. [DOI] [PubMed] [Google Scholar]
  6. Griffin N. R., Bevan I. S., Lewis F. A., Wells M., Young L. S. Demonstration of multiple HPV types in normal cervix and in cervical squamous cell carcinoma using the polymerase chain reaction on paraffin wax embedded material. J Clin Pathol. 1990 Jan;43(1):52–56. doi: 10.1136/jcp.43.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ji H. X., Syrjänen S., Syrjänen K., Wu A. R., Chang F. J. In situ hybridization analysis of HPV DNA in cervical precancer and cervical cancers from China. Arch Gynecol Obstet. 1990;247(1):21–29. doi: 10.1007/BF02390651. [DOI] [PubMed] [Google Scholar]
  8. Kinlen L. J., Spriggs A. I. Women with positive cervical smears but without surgical intervention. A follow-up study. Lancet. 1978 Aug 26;2(8087):463–465. doi: 10.1016/s0140-6736(78)91457-5. [DOI] [PubMed] [Google Scholar]
  9. Kiyabu M. T., Shibata D., Arnheim N., Martin W. J., Fitzgibbons P. L. Detection of human papillomavirus in formalin-fixed, invasive squamous carcinomas using the polymerase chain reaction. Am J Surg Pathol. 1989 Mar;13(3):221–224. doi: 10.1097/00000478-198903000-00007. [DOI] [PubMed] [Google Scholar]
  10. Kurman R. J., Schiffman M. H., Lancaster W. D., Reid R., Jenson A. B., Temple G. F., Lorincz A. T. Analysis of individual human papillomavirus types in cervical neoplasia: a possible role for type 18 in rapid progression. Am J Obstet Gynecol. 1988 Aug;159(2):293–296. doi: 10.1016/s0002-9378(88)80070-x. [DOI] [PubMed] [Google Scholar]
  11. Matlashewski G. The cell biology of human papillomavirus transformed cells. Anticancer Res. 1989 Sep-Oct;9(5):1447–1456. [PubMed] [Google Scholar]
  12. McCance D. J., Campion M. J., Clarkson P. K., Chesters P. M., Jenkins D., Singer A. Prevalence of human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and invasive carcinoma of the cervix. Br J Obstet Gynaecol. 1985 Nov;92(11):1101–1105. doi: 10.1111/j.1471-0528.1985.tb03019.x. [DOI] [PubMed] [Google Scholar]
  13. Meanwell C. A., Cox M. F., Blackledge G., Maitland N. J. HPV 16 DNA in normal and malignant cervical epithelium: implications for the aetiology and behaviour of cervical neoplasia. Lancet. 1987 Mar 28;1(8535):703–707. doi: 10.1016/s0140-6736(87)90353-9. [DOI] [PubMed] [Google Scholar]
  14. Mincheva A., Gissmann L., zur Hausen H. Chromosomal integration sites of human papillomavirus DNA in three cervical cancer cell lines mapped by in situ hybridization. Med Microbiol Immunol. 1987;176(5):245–256. doi: 10.1007/BF00190531. [DOI] [PubMed] [Google Scholar]
  15. Riou G., Favre M., Jeannel D., Bourhis J., Le Doussal V., Orth G. Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA. Lancet. 1990 May 19;335(8699):1171–1174. doi: 10.1016/0140-6736(90)92693-c. [DOI] [PubMed] [Google Scholar]
  16. Schmauz R., Okong P., de Villiers E. M., Dennin R., Brade L., Lwanga S. K., Owor R. Multiple infections in cases of cervical cancer from a high-incidence area in tropical Africa. Int J Cancer. 1989 May 15;43(5):805–809. doi: 10.1002/ijc.2910430511. [DOI] [PubMed] [Google Scholar]
  17. Scholl S. M., Pillers E. M., Robinson R. E., Farrell P. J. Prevalence of human papillomavirus type 16 DNA in cervical carcinoma samples in East Anglia. Int J Cancer. 1985 Feb 15;35(2):215–218. doi: 10.1002/ijc.2910350212. [DOI] [PubMed] [Google Scholar]
  18. Shibata D., Fu Y. S., Gupta J. W., Shah K. V., Arnheim N., Martin W. J. Detection of human papillomavirus in normal and dysplastic tissue by the polymerase chain reaction. Lab Invest. 1988 Oct;59(4):555–559. [PubMed] [Google Scholar]
  19. Wilczynski S. P., Bergen S., Walker J., Liao S. Y., Pearlman L. F. Human papillomaviruses and cervical cancer: analysis of histopathologic features associated with different viral types. Hum Pathol. 1988 Jun;19(6):697–704. doi: 10.1016/s0046-8177(88)80176-x. [DOI] [PubMed] [Google Scholar]

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