Abstract
The dataset described herein is related to our article entitled “T-cell antigens of Mycobacterium immunogenum (MI), an etiological agent of occupational hypersensitivity pneumonitis’’ (Chandra and Yadav, 2016) [1]. The data include in silico-predicted T-cell epitopes of the T-cell antigens AgA and AgD of MI predicted to bind to HLA-I or HLA-II alleles. Data on two reference T-cell antigens ESAT-6 and CFP-10 of Mycobacterium tuberculosis H37Rv are included for comparison. The data for each antigen include the predicted epitope׳s amino acid sequence, its first amino acid position, and its ability to bind HLA-I or HLA-II allele(s).
Keywords: Immunoinformatics, T-cell antigen, T-cell epitopes, HLA-I, HLA-II, Mycobacterium immunogenum, Mycobacterium tuberculosis, Hypersensitivity pneumonitis
Specifications Table
| Subject area | Immunology |
| More specific subject area | Immunoinformatics |
| Type of data | Table |
| How data was acquired | In silico analysis of the T-cell antigens for epitopes |
| Data format | Analyzed |
| Experimental factors | The antigens were originally identified by 2D-immunoproteomics |
| Experimental features | T-cell reactive antigens were subjected to in silico analysis for HLA-I and HLA-II binding epitopes |
| Data source location | University of Cincinnati, College of Medicine, Cincinnati, OH, USA |
| Data accessibility | All relevant data provided in this article |
Value of the data
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Occupational hypersensitivity pneumonitis (HP) is a T-cell-mediated disease caused by exposure to mycobacterial antigens via machining fluids contaminated with Mycobacterium immunogenum (MI). Currently there is no data on epitopes of this pathogen.
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The current data set relates to the first report on epitope prediction for the first set of T-cell antigens of this HP etiological agent.
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Knowledge of the T-cell epitopes which bind to HLA-I and HLA-II alleles in human population is significant as it can provide the needed information to develop further diagnostic tools and therapeutic or vaccine targets. HP is still a difficult-to-diagnose occupational lung disease due to the lack of such data.
1. Data
The dataset presented in this article is about epitopes of newly identified T-cell antigens of Mycobacterium immunogenum (MI) that causes HP [1], [2], [3]. The data show predicted HLA-I alleles for T-cell epitopes of AgA and AgD of MI (Tables 1 and 2) and ESAT-6 and CFP-10 of M. tuberculosis H37Rv (Tables 5 and 6), respectively. The corresponding data for predicted HLA-II alleles are presented in Tables 3 and 4 (MI antigens) and Tables 7 and 8 (reference antigens of M. tuberculosis), respectively.
2. Experimental design, materials and methods
The data in this article focus on two recombinant MI antigen proteins, AgA and AgD, selected based on the in vitro T-cell reactivity [1] from a pool of 33 seroreactive proteins of M. immunogenum originally identified in our previous 2D- immunoproteomics work [4], [5] . The selected antigens AgA and AgD were analyzed here for epitopes using in silico immunoinformatic approach. Amino acid sequences of the antigens were used for the in silico analysis. For comparison, two well-recognized immunodominant antigens ESAT-6 and CFP-10 of the tuberculosis agent M. tuberculosis were analyzed in parallel. The epitopes were predicted using ProPred-I and ProPred platforms that are meant to predict promiscuous binding regions for 47 and 51 different HLA class I and class II alleles, respectively [6].
Acknowledgements
This study was supported by Grant 2R01OH007364 (to JSY) from the National Institute for Occupational Safety and Health (NIOSH), the Centers for Disease Control and Prevention (CDC) (Grant: 2R01OH007364), USA.
Footnotes
Transparency data associated with this article can be found in the online version at http://dx.doi.org/10.1016/j.dib.2016.06.045.
Supplementary data associated with this article can be found in the online version at http://dx.doi.org/10.1016/j.dib.2016.06.045.
Transparency document. Supplementary material
Supplementary material
Appendix A. Supplementary material
Supplementary material
References
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Associated Data
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Supplementary Materials
Supplementary material
Supplementary material