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. 1991 Jan;44(1):25–28. doi: 10.1136/jcp.44.1.25

Evaluation of the pre-S (pre-S(1)Ag/pre-S(2)Ab) system in hepatitis B virus infection.

M I Galán 1, J Tomás 1, M C Bernal 1, F J Salmerón 1, M C Maroto 1
PMCID: PMC497009  PMID: 1997531

Abstract

The diagnostic and prognostic value of pre-S(1)Ag and pre-S(2)Ab was investigated in 69 HBsAg surface antigen positive patients--14 with acute hepatitis B, 30 with chronic liver disease (six chronic persistent hepatitis, 14 chronic active hepatitis, 10 with cirrhosis) and in 25 asymptomatic carriers. Pre-S(1)Ag was found in all patients with chronic hepatitis B virus (HBV) infection regardless of viral replication. In contrast, pre-S(2)Ab was not detected in any patients. Acute hepatitis was studied sequentially with periodic controls at 20 day intervals. Pre-S(1)Ag cleared before HBsAg in six of 14 (43%) patients who progressed favourably, and the two antigens cleared simultaneously in eight of 14 (57%) cases. Patients with early clearance of pre-S(1)Ag progressed favourably, thus indicating the prognostic value of this test, which, however, is still of limited practical application given the small temporal difference between the moment of clearance of the two antigens. The first markers to clear, however, were HBeAg and DNA-HBV, which showed significant differences with respect to the clearance of HBsAg. Moreover, pre-S(2)Ab appeared before HBsAb in 57.1% of our patients and was found in some patients before pre-S(1)Ag and HBsAg had cleared (42.8%), thus allowing complete viral clearance and acute HBV infection to be predicted earlier.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberti A., Cavalletto D., Pontisso P., Chemello L., Tagariello G., Belussi F. Antibody response to pre-S2 and hepatitis B virus induced liver damage. Lancet. 1988 Jun 25;1(8600):1421–1424. doi: 10.1016/s0140-6736(88)92237-4. [DOI] [PubMed] [Google Scholar]
  2. Budkowska A., Briantais M. J., Dubreuil P., Capel F., Grangeot-Keros L., Pillot J. Detection of antibodies directed against the pre-S gene product of hepatitis B virus: relationship between anti-pre-S response and recovery. Ann Inst Pasteur Immunol. 1985 Jul-Aug;136D(1):57–65. doi: 10.1016/s0769-2625(85)80075-1. [DOI] [PubMed] [Google Scholar]
  3. Budkowska A., Dubreuil P., Capel F., Pillot J. Hepatitis B virus pre-S gene-encoded antigenic specificity and anti-pre-S antibody: relationship between anti-pre-S response and recovery. Hepatology. 1986 May-Jun;6(3):360–368. doi: 10.1002/hep.1840060305. [DOI] [PubMed] [Google Scholar]
  4. Hadziyannis S., Raimondo G., Papaioannou C., Anastassakos C., Wong D., Sninsky J., Shafritz D. Expression of pre-S gene-encoded proteins in liver and serum during chronic hepatitis B virus infection in comparison to other markers of active virus replication. J Hepatol. 1987 Dec;5(3):253–259. doi: 10.1016/s0168-8278(87)80029-6. [DOI] [PubMed] [Google Scholar]
  5. Hellström U., Sylvan S., Kuhns M., Sarin V. Absence of pre-S2 antibodies in natural hepatitis B virus infection. Lancet. 1986 Oct 18;2(8512):889–893. doi: 10.1016/s0140-6736(86)90412-5. [DOI] [PubMed] [Google Scholar]
  6. Hess G., Gerken G., Weber C., Knolle J., Dormeyer H. H., Arnold W., Manns M., Meyer zum Büschenfelde K. H. Detection of hepatitis B virus markers in sera of asymptomatic hepatitis B surface antigen carriers with special emphasis to pre-S-encoded proteins. Digestion. 1987;38(2):90–95. doi: 10.1159/000199577. [DOI] [PubMed] [Google Scholar]
  7. Klinkert M. Q., Theilmann L., Pfaff E., Schaller H. Pre-S1 antigens and antibodies early in the course of acute hepatitis B virus infection. J Virol. 1986 May;58(2):522–525. doi: 10.1128/jvi.58.2.522-525.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Krogsgaard K., Aldershvile J., Kryger P., Andersson P., Nielsen J. O., Hansson B. G. Hepatitis B virus DNA, HBeAg and delta infection during the course from acute to chronic hepatitis B virus infection. Hepatology. 1985 Sep-Oct;5(5):778–782. doi: 10.1002/hep.1840050512. [DOI] [PubMed] [Google Scholar]
  9. Krogsgaard K., Kryger P., Aldershvile J., Andersson P., Brechot C. Hepatitis B virus DNA in serum from patients with acute hepatitis B. Hepatology. 1985 Jan-Feb;5(1):10–13. doi: 10.1002/hep.1840050104. [DOI] [PubMed] [Google Scholar]
  10. Machida A., Ohnuma H., Takai E., Tanaka T., Itoh Y., Tsuda F., Akahane Y., Usuda S., Nakamura T., Miyakawa Y. A synthetic peptide coded for by the pre-S2 region of hepatitis B virus for adding immunogenicity to small spherical particles made of the product of the S gene. Mol Immunol. 1987 May;24(5):523–529. doi: 10.1016/0161-5890(87)90027-7. [DOI] [PubMed] [Google Scholar]
  11. Meyer zum Büschenfelde K. H., Gerken G., Hess G., Manns M. The significance of the pre-S region of the hepatitis B virus. J Hepatol. 1986;3(2):273–279. doi: 10.1016/s0168-8278(86)80038-1. [DOI] [PubMed] [Google Scholar]
  12. Picazo de la Garza J. J. Las proteínas pre-S en la hepatitis B. Rev Clin Esp. 1987 Nov;181(8):453–457. [PubMed] [Google Scholar]
  13. Steward M. W., Sisley B. M., Stanley C., Brown S. E., Howard C. R. Immunity to hepatitis B: analysis of antibody and cellular responses in recipients of a plasma-derived vaccine using synthetic peptides mimicking S and pre-S regions. Clin Exp Immunol. 1988 Jan;71(1):19–25. [PMC free article] [PubMed] [Google Scholar]
  14. Stibbe W., Gerlich W. H. Characterization of pre-s gene products in hepatitis B surface antigen. Dev Biol Stand. 1983;54:33–43. [PubMed] [Google Scholar]
  15. Theilmann L., Klinkert M. Q., Gmelin K., Kommerell B., Pfaff E. Detection of antibodies against pre-S1 proteins in sera of patients with hepatitis B virus (HBV) infection. J Hepatol. 1987 Feb;4(1):22–28. doi: 10.1016/s0168-8278(87)80005-3. [DOI] [PubMed] [Google Scholar]
  16. Theilmann L., Klinkert M. Q., Gmelin K., Salfeld J., Schaller H., Pfaff E. Detection of pre-S1 proteins in serum and liver of HBsAg-positive patients: a new marker for hepatitis B virus infection. Hepatology. 1986 Mar-Apr;6(2):186–190. doi: 10.1002/hep.1840060206. [DOI] [PubMed] [Google Scholar]
  17. Wood J. R., Taswell H. F., Czaja A. J., Rabe D. Pattern and duration of HBV DNA seropositivity in acute hepatitis B. Dig Dis Sci. 1988 Apr;33(4):477–480. doi: 10.1007/BF01536035. [DOI] [PubMed] [Google Scholar]

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