Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1991 Jan;44(1):29–32. doi: 10.1136/jcp.44.1.29

Endothelial markers in malignant vascular tumours of the liver: superiority of QB-END/10 over von Willebrand factor and Ulex europaeus agglutinin 1.

P P Anthony 1, P Ramani 1
PMCID: PMC497010  PMID: 1705261

Abstract

A new monoclonal antibody, QB-END/10, raised against the CD34 antigen in human endothelial cell membranes and haemopoietic progenitor cells, was studied for its usefulness as a marker of neoplastic vascular cells in 21 angiosarcomas and seven malignant haemangioendotheliomas of the liver. QB-END/10 was both more sensitive and more specific than Von Willebrand factor (VWF) and Ulex europaeus 1 agglutinin (UEA-1) in labelling endothelial cells and it did not cross react with epithelia as UEA-1 often does. Staining was uniformly strong and clear in all histological variants of these two tumours. QB-END/10 should prove particularly useful in the differential diagnosis of malignant vascular tumours of the liver.

Full text

PDF
29

Images in this article

30Image
on p.30
30Image
on p.30
31Image
on p.31
31Image
on p.31

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alles J. U., Bosslet K. Immunocytochemistry of angiosarcomas. A study of 19 cases with special emphasis on the applicability of endothelial cell specific markers to routinely prepared tissues. Am J Clin Pathol. 1988 Apr;89(4):463–471. doi: 10.1093/ajcp/89.4.463. [DOI] [PubMed] [Google Scholar]
  2. Baxter P. J., Anthony P. P., MacSween R. N., Scheuer P. J. Angiosarcoma of the liver in Great Britain, 1963-73. Br Med J. 1977 Oct 8;2(6092):919–921. doi: 10.1136/bmj.2.6092.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berry C. L., Amerigo J. Blood group antigens in vascular tumours. Evaluation of the immunoperoxidase technique. Virchows Arch A Pathol Anat Histol. 1980;388(2):167–174. doi: 10.1007/BF00430685. [DOI] [PubMed] [Google Scholar]
  4. Bukh A., Ingerslev J., Stenbjerg S., Møller N. P. The multimeric structure of plasma F VIII:RAg studied by electroelution and immunoperoxidase detection. Thromb Res. 1986 Sep 1;43(5):579–584. doi: 10.1016/0049-3848(86)90078-2. [DOI] [PubMed] [Google Scholar]
  5. Corrin B., Manners B., Millard M., Weaver L. Histogenesis of the so-called "intravascular bronchioloalveolar tumour". J Pathol. 1979 Jul;128(3):163–167. doi: 10.1002/path.1711280308. [DOI] [PubMed] [Google Scholar]
  6. Dietze O., Davies S. E., Williams R., Portmann B. Malignant epithelioid haemangioendothelioma of the liver: a clinicopathological and histochemical study of 12 cases. Histopathology. 1989 Sep;15(3):225–237. doi: 10.1111/j.1365-2559.1989.tb03073.x. [DOI] [PubMed] [Google Scholar]
  7. Fina L., Molgaard H. V., Robertson D., Bradley N. J., Monaghan P., Delia D., Sutherland D. R., Baker M. A., Greaves M. F. Expression of the CD34 gene in vascular endothelial cells. Blood. 1990 Jun 15;75(12):2417–2426. [PubMed] [Google Scholar]
  8. Hultberg B. M., Daugaard S., Johansen H. F., Mouridsen H. T., Hou-Jensen K. Malignant haemangiopericytomas and haemangioendotheliosarcomas: an immunohistochemical study. Histopathology. 1988 Apr;12(4):405–414. doi: 10.1111/j.1365-2559.1988.tb01955.x. [DOI] [PubMed] [Google Scholar]
  9. Ishak K. G., Sesterhenn I. A., Goodman Z. D., Rabin L., Stromeyer F. W. Epithelioid hemangioendothelioma of the liver: a clinicopathologic and follow-up study of 32 cases. Hum Pathol. 1984 Sep;15(9):839–852. doi: 10.1016/s0046-8177(84)80145-8. [DOI] [PubMed] [Google Scholar]
  10. Little D., Said J. W., Siegel R. J., Fealy M., Fishbein M. C. Endothelial cell markers in vascular neoplasms: an immunohistochemical study comparing factor VIII-related antigen, blood group specific antigens, 6-keto-PGF1 alpha, and Ulex europaeus 1 lectin. J Pathol. 1986 Jun;149(2):89–95. doi: 10.1002/path.1711490203. [DOI] [PubMed] [Google Scholar]
  11. Miettinen M., Holthofer H., Lehto V. P., Miettinen A., Virtanen I. Ulex europaeus I lectin as a marker for tumors derived from endothelial cells. Am J Clin Pathol. 1983 Jan;79(1):32–36. doi: 10.1093/ajcp/79.1.32. [DOI] [PubMed] [Google Scholar]
  12. Mukai K., Rosai J., Burgdorf W. H. Localization of factor VIII-related antigen in vascular endothelial cells using an immunoperoxidase method. Am J Surg Pathol. 1980 Jun;4(3):273–276. doi: 10.1097/00000478-198006000-00008. [DOI] [PubMed] [Google Scholar]
  13. Scheuer P. J. Changing views on chronic hepatitis. Histopathology. 1986 Jan;10(1):1–4. doi: 10.1111/j.1365-2559.1986.tb02455.x. [DOI] [PubMed] [Google Scholar]
  14. Selinger M., Koff R. S. Thorotrast and the liver: a reminder. Gastroenterology. 1975 Apr;68(4 Pt 1):799–803. [PubMed] [Google Scholar]
  15. Stephenson T. J., Mills P. M. Monoclonal antibodies to blood group isoantigens: an alternative marker to factor VIII related antigen for benign and malignant vascular endothelial cells. J Pathol. 1985 Oct;147(2):139–148. doi: 10.1002/path.1711470210. [DOI] [PubMed] [Google Scholar]
  16. Sutherland D. R., Watt S. M., Dowden G., Karhi K., Baker M. A., Greaves M. F., Smart J. E. Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34. Leukemia. 1988 Dec;2(12):793–803. [PubMed] [Google Scholar]
  17. Torrado J., Blasco E., Cosme A., Gutierrez-Hoyos A., Arenas J. I. Expression of type 1 and type 2 blood group-related antigens in normal and neoplastic gastric mucosa. Am J Clin Pathol. 1989 Mar;91(3):249–254. doi: 10.1093/ajcp/91.3.249. [DOI] [PubMed] [Google Scholar]
  18. Watt S. M., Karhi K., Gatter K., Furley A. J., Katz F. E., Healy L. E., Altass L. J., Bradley N. J., Sutherland D. R., Levinsky R. Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hemopoietic progenitor cells. Leukemia. 1987 May;1(5):417–426. [PubMed] [Google Scholar]
  19. Weiss S. W., Enzinger F. M. Epithelioid hemangioendothelioma: a vascular tumor often mistaken for a carcinoma. Cancer. 1982 Sep 1;50(5):970–981. doi: 10.1002/1097-0142(19820901)50:5<970::aid-cncr2820500527>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  20. Yonezawa S., Maruyama I., Sakae K., Igata A., Majerus P. W., Sato E. Thrombomodulin as a marker for vascular tumors. Comparative study with factor VIII and Ulex europaeus I lectin. Am J Clin Pathol. 1987 Oct;88(4):405–411. doi: 10.1093/ajcp/88.4.405. [DOI] [PubMed] [Google Scholar]
  21. Zafrani E. S. Update on vascular tumours of the liver. J Hepatol. 1989 Jan;8(1):125–130. doi: 10.1016/0168-8278(89)90171-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES