Fig. 5.

Downregulation of MEIS1 causes MPNSTs cell cycle arrest in G1 and cell death. (A) Phase contrast images show fewer cells on plates infected with shMEIS1 versus shNT day 6 post infections. (B) Flow cytometry data for PI and AnnexinV stained S462-TY cells transduced with shControl or shMEIS1 4.5 days p.i. (C) Histogram displays data from flow cytometry analysis using the DNA dye propidium iodide (P.I.) showing the cell cycle phases including G1, S and G2. ShMEIS1 treated samples (red line) show fewer cells in S-phase and a significant increase in percent dead cells versus untreated (UN) or shNT infected cells all at post infection day 4.5. (D) Immunoblots show levels of cell cycle proteins p27^Kip, phospho-Rb at designated phosphorylation sites, cyclinE2 and cyclinD1cell p.i day 5 in UN, shNT and shMEIS1 MPNST cells. Cleaved PARP marks cell death. Anti-β-actin was used as a loading control.