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. 1982 Feb;35(2):139–143. doi: 10.1136/jcp.35.2.139

Detection of surface immunoglobulins of human lymphoid cells: a comparative study of live and fixed cells using a direct immunoperoxidase procedure.

G Laurent, M F Gourdin, F Reyes
PMCID: PMC497486  PMID: 7040480

Abstract

Surface immunoglobulins (Ig) of normal and malignant lymphoid cells were detected on prefixed, smeared (method A) and live (method B) cell suspensions; the results were compared with regard to staining patterns, specificity and sensitivity. In both methods surface Ig were detected by a direct immunoperoxidase procedure using conjugated purified antibody. Although method A has practical advantages, method B is more sensitive. The reasons for this discrepancy are discussed in relation to surface Ig denaturation and redistribution.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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