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. 1992 Aug 15;89(16):7576–7580. doi: 10.1073/pnas.89.16.7576

Purification and characterization of the diphtheria toxin repressor.

M P Schmitt 1, E M Twiddy 1, R K Holmes 1
PMCID: PMC49753  PMID: 1502169

Abstract

The diphtheria toxin repressor gene (dtxR) encodes a protein (DtxR) that regulates transcription of the diphtheria toxin gene (tox) by an iron-dependent mechanism. Cloned dtxR was expressed in Escherichia coli from the phage T7 gene 10 promoter, and DtxR was purified. Specific binding of DtxR to the tox+ operator was dependent on reduction of DtxR and the presence of ferrous ions. DtxR protected a sequence of approximately 30 nucleotide pairs, partially overlapping the tox promoter and containing a region of dyad symmetry, from digestion by DNase I. DtxR exhibited very little binding to the mutant tox-201 operator region and failed to bind to the promoter/operator region of the ferric uptake regulation (fur) gene of E. coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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