Abstract
Indirect immunofluorescence studies were performed using sera and IgG-Fab2 fragments from patients with chronic active hepatitis (CAH) who were positive for a liver membrane antibody (LMA). The specificity was investigated using hepatocytes from humans as well as rabbit, rat, guinea pig and monkey. Only sera also positive for smooth muscle antibody gave staining of lymphocytes and absorption with F-actin from rabbit muscle abolished this as well as all other smooth muscle staining without influencing LMA. It was concluded that LMA, routinely detected by indirect immunofluorescence using rabbit hepatocytes, represents specific binding to non-species-specific membrane antigens which are normal constituents of human hepatocytes. The antigen is separately located, and not cross-reactive with F-actin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOYUM A. SEPARATION OF WHITE BLOOD CELLS. Nature. 1964 Nov 21;204:793–794. doi: 10.1038/204793a0. [DOI] [PubMed] [Google Scholar]
- Gluud C., Tage-Jensen U., Bahnsen M., Dietrichson O., Svejgaard A. Autoantibodies, histocompatibility antigens and testosterone in males with alcoholic liver cirrhosis. Clin Exp Immunol. 1981 Apr;44(1):31–37. [PMC free article] [PubMed] [Google Scholar]
- Hopf U., Meyer zum Büschenfelde K. H., Arnold W. Detection of a liver-membrane autoantibody in HBsAg-negative chronic active hepatitis. N Engl J Med. 1976 Mar 11;294(11):578–582. doi: 10.1056/NEJM197603112941103. [DOI] [PubMed] [Google Scholar]
- Krogsgaard K., Tage-Jensen U., Wantzin P., Aldershvile J., Hardt F. Localisation of immunoglobulin on the liver cell surface in primary biliary cirrhosis. J Clin Pathol. 1981 Oct;34(10):1076–1079. doi: 10.1136/jcp.34.10.1076. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lidman K., Biberfeld G., Fagraeus A., Norberg R., Torstensson R., Utter G., Carlsson L., Luca J., Lindberg U. Anti-actin specificity of human smooth muscle antibodies in chronic active hepatitis. Clin Exp Immunol. 1976 May;24(2):266–272. [PMC free article] [PubMed] [Google Scholar]
- Manthorpe R., Permin H., Tage-Jensen U. Auto-antibodies in Sjögren's syndrome. With special reference to liver-cell membrane antibody (LMA). Scand J Rheumatol. 1979;8(3):168–172. doi: 10.3109/03009747909114450. [DOI] [PubMed] [Google Scholar]
- Meyer zum Büschenfelde K. H., Manns M., Hütteroth T. H., Hopf U., Arnold W. LM-Ag and LSP--two different target antigens involved in the immunopathogenesis of chronic active hepatitis? Clin Exp Immunol. 1979 Aug;37(2):205–212. [PMC free article] [PubMed] [Google Scholar]
- Meyer zum Büschenfelde, Miescher P. A. Liver specific antigens. Purification and characterization. Clin Exp Immunol. 1972 Jan;10(1):89–102. [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Tage-Jensen U., Arnold W., Dietrichson O., Hardt F., Hopf U., Meyer Zum Büschenfelde K. H., Nielsen J. O. Liver-cell-membrane autoantibody specific for inflammatory liver diseases. Br Med J. 1977 Jan 22;1(6055):206–208. doi: 10.1136/bmj.1.6055.206. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tage-Jensen U., Permin H., Hardt F., Juhl E., Mathiesen L. R., Nielsen J. O., Ranek L. Circulating autoantibodies in patients with acute viral hepatitis. Relation to etiology and clinical course. Scand J Gastroenterol. 1980;15(2):229–235. doi: 10.3109/00365528009181460. [DOI] [PubMed] [Google Scholar]
- Trenchev P., Sneyd P., Holborow E. J. Immunofluorescent tracing of smooth muscle contractile protein antigens in tissues other than smooth muscle. Clin Exp Immunol. 1974 Jan;16(1):125–135. [PMC free article] [PubMed] [Google Scholar]