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Three-dimensional (3D) microtissue derived from primary thyroid cells. (A) The 3D spheroid formed by hanging-drop system and pictured on day 21. An arrow shows the 3D microtissue under a microscope with 50 × magnification. (B) Thyroid marker gene expression profile of the 3D microtissue. GAPDH was used as a housekeeping control. RT indicates a reverse transcription reaction with reverse transcriptase; –RT indicates a reaction without reverse transcriptase.
