Abstract
The protein contents and binding modes of immunoglobulin-amylase complexes were investigated. Amylase staining after immunoelectrophoresis permitted identification of IgA-lambda type in three cases, IgA-kappa type in two cases and IgG-kappa type in one case. The precipitin line of Fab fragment of IgA in four patient's sera after papain digestion was found to have amylase activity. Sucrose density gradient ultracentrifugation studies showed that these complexes were divided into three groups: Group I complexes were dissociated in acid pH, and at pH 7.0 the immunoglobulins, which were isolated at the acid pH, became rebound to serum amylase; Group II complexes were dissociated by acidification, but did not reform at pH 7.0; Group III complexes were unaffected by acidification. We suggest that the immunoglobulin-amylase complexes belonging to groups I and II result from an antigen-antibody reaction. Precipitin curves resulting from the addition of increasing quantities of the patient's serum containing 9 S or 11 S immunoglobulin-amylase complexes to specific antisera revealed that these complexes contained not only monoclonal IgA, but also alpha-1-antitrypsin (alpha 1 AT) and albumin. These IgA complexes could have bound non-specifically to alpha 1 AT and albumin, forming the larger macroamylase complexes.
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- Berk J. E., Kizu H., Wilding P., Searcy R. L. Macroamylasemia: a newly recognized cause for elevated serum amylase activity. N Engl J Med. 1967 Nov 2;277(18):941–946. doi: 10.1056/NEJM196711022771801. [DOI] [PubMed] [Google Scholar]
- Grey H. M., Abel C. A., Yount W. J., Kunkel H. G. A subclass of human gamma-A-globulins (gamma-A2) which lacks the disulfied bonds linking heavy and light chains. J Exp Med. 1968 Dec 1;128(6):1223–1236. doi: 10.1084/jem.128.6.1223. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hansen H. R., Van Kley H., Knight W. A., Jr Macroamylasemia due to binding by protein. Am J Med. 1972 May;52(5):712–720. doi: 10.1016/0002-9343(72)90062-9. [DOI] [PubMed] [Google Scholar]
- Ishizaka K., Ishizaka T., Lee E. H. Immunochemical properties of human gamma A isoagglutinin. II. The effect of reduction and alkylation. J Immunol. 1965 Nov;95(5):771–780. [PubMed] [Google Scholar]
- Köhler H., Kirch W., Weihrauch T. R., Prellwitz W., Horstmann H. J. Macroamylasaemia after treatment with hydroxyethyl starch. Eur J Clin Invest. 1977 Jun;7(3):205–211. doi: 10.1111/j.1365-2362.1977.tb01599.x. [DOI] [PubMed] [Google Scholar]
- Levitt M. D., Cooperband S. R. Hyperamylasemia from the binding of serum amylase by an 11S IgA globulin. N Engl J Med. 1968 Feb 29;278(9):474–479. doi: 10.1056/NEJM196802292780903. [DOI] [PubMed] [Google Scholar]
- Levitt M. D., Goetzl E. J., Cooperband S. R. Two forms of macroamylasaemia. Lancet. 1968 May 4;1(7549):957–958. doi: 10.1016/s0140-6736(68)90907-0. [DOI] [PubMed] [Google Scholar]
- Mannik M. Binding of albumin to gamma-A-myeloma proteins and Waldenström macroglobulins by disulfide bonds. J Immunol. 1967 Nov;99(5):899–906. [PubMed] [Google Scholar]
- PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Take S., Fridhandler L., Berk J. E. Macroamylasemia: possible role of polysaccharide in composition of macroamylase. Clin Chim Acta. 1970 Feb;27(2):369–371. doi: 10.1016/0009-8981(70)90364-5. [DOI] [PubMed] [Google Scholar]
- Tomasi T. B., Jr, Hauptman S. P. The binding of alpha-1 antitrypsin to human IgA. J Immunol. 1974 Jun;112(6):2274–2277. [PubMed] [Google Scholar]
- Ueda M., Berk J. E., Fridhandler L., Davis J. Ultracentrifugal characteristics of macroamylasemic serum. Clin Chim Acta. 1971 Dec;35(2):299–304. doi: 10.1016/0009-8981(71)90197-5. [DOI] [PubMed] [Google Scholar]
- Ueda M., Berk J. E., Fridhandler L. Macroamylasemia: variation in the response of the macroamylase complex to acidification. Proc Soc Exp Biol Med. 1971 Sep;137(4):1152–1156. doi: 10.3181/00379727-137-35745. [DOI] [PubMed] [Google Scholar]