Abstract
The histochemical creatine phosphokinase (CPK) tetrazolium test has been evaluated to detect recent human myocardial infarction in gross slices of the heart at necropsy. The demonstration of the lesion with this method has been assumed to result from local loss of CPK from the damaged myocardium. However, the present study indicates that the mechanism involved depends on localising NADPH tetrazolium reductase and not CPK. Phenazine methosulphate (PMS), when added to the incubating medium as an electron-acceptor to circumvent the tetrazolium-reductase (diaphorase) system, resulted in generalised false staining of the heart slice.
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- Anderson K. R., Popple A., Parker D. J., Sayer R., Trickey R. J., Davies M. J. An experimental assessment of macroscopic enzyme techniques for the autopsy demonstration of myocardial infarction. J Pathol. 1979 Feb;127(2):93–98. doi: 10.1002/path.1711270207. [DOI] [PubMed] [Google Scholar]
- Derias N. W., Adams C. W. Macroscopic enzyme histochemistry in myocardial infarction: use of coenzyme, cyanide, and phenazine methosulphate. J Clin Pathol. 1982 Apr;35(4):410–413. doi: 10.1136/jcp.35.4.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Derias N. W., Adams C. W. Nitroblue tetrazolium test: early gross detection of human myocardial infarcts. Br J Exp Pathol. 1978 Jun;59(3):254–258. [PMC free article] [PubMed] [Google Scholar]
- HESS J. W., MACDONALD R. P., FREDERICK R. J., JONES R. N., NEELY J., GROSS D. SERUM CREATINE PHOSPHOKINASE (CPK) ACTIVITY IN DISORDERS OF HEART AND SKELETAL MUSCLE. Ann Intern Med. 1964 Dec;61:1015–1028. doi: 10.7326/0003-4819-61-6-1015. [DOI] [PubMed] [Google Scholar]
- Sjövall K. A tetrazolium technique for the histochemical localization of ATP: creatine phosphotransferase. Pattern in rat and human skeletal muscle. Histochemie. 1967;10(4):336–340. doi: 10.1007/BF00304316. [DOI] [PubMed] [Google Scholar]