Abstract
We have measured serum immunoreactive trypsin (IRT) and serum pancreatic isoamylase (PIA) activities using commercially available kits in 37 cystic fibrosis (CF) patients and 46 hospital controls of similar age range. Immunoreactive trypsin was more often abnormal than PIA (26/37 v 18/37 abnormal respectively); IRT will be particularly useful as an additional diagnostic test in older children, in whom interpretation of the sweat test may be difficult, as 14/15 CF patients aged over 10 years had abnormal IRT results. Less than half of our patients who were aged between one and nine years had abnormal IRT activity, limiting the value of the test, though a low activity would still support the diagnosis of CF. Comparison with faecal fat estimations in 31 patients suggests that neither IRT nor PIA can be used as a non-invasive test of pancreatic function in order to identify those few CF patients who do not require pancreatic enzyme supplements.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burlina A., Rizzotti P., Tonon M., Mastella G. Serum immunoreactive trypsin in cystic fibrosis. Scand J Gastroenterol Suppl. 1980;62:35–43. [PubMed] [Google Scholar]
- Dandona P., Hodson M., Bell J., Ramdial L., Beldon I., Batten J. C. Serum immunoreactive trypsin in cystic fibrosis. Thorax. 1981 Jan;36(1):60–62. doi: 10.1136/thx.36.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis P. B., Hubbard V. S., Di Sant'Agnese P. A. Low sweat electrolytes in a patient with cystic fibrosis. Am J Med. 1980 Oct;69(4):643–646. doi: 10.1016/0002-9343(80)90482-9. [DOI] [PubMed] [Google Scholar]
- Gillard B. K., Simbala J. A., Feig S. A. Serum amylase isoenzymes in cystic fibrosis patients. Evidence for a generalized defect in exocrine gland secretory regulation. Pediatr Res. 1980 Nov;14(11):1168–1172. doi: 10.1203/00006450-198011000-00004. [DOI] [PubMed] [Google Scholar]
- Kenny D., Cooke A., Tempany E., McGeeney K. F. Activity of serum alpha-amylases in cystic fibrosis. Clin Chim Acta. 1978 Nov 1;89(3):429–433. doi: 10.1016/0009-8981(78)90406-0. [DOI] [PubMed] [Google Scholar]
- King D. N., Heeley A. F., Walsh M. P., Kuzemko J. A. Sensitive trypsin assay for dried-blood specimens as a screening procedure for early detection of cystic fibrosis. Lancet. 1979 Dec 8;2(8154):1217–1219. doi: 10.1016/s0140-6736(79)92336-5. [DOI] [PubMed] [Google Scholar]
- O'Donnell M. D., FitzGerald O., McGeeney K. F. Differential serum amylase determination by use of an inhibitor, and design of a routine procedure. Clin Chem. 1977 Mar;23(3):560–566. [PubMed] [Google Scholar]
- Skude G., Kollberg H. Serum isoamylases in cystic fibrosis. Acta Paediatr Scand. 1976 Mar;65(2):145–149. doi: 10.1111/j.1651-2227.1976.tb16527.x. [DOI] [PubMed] [Google Scholar]
- Smalley C. A., Addy D. P., Anderson C. M. Does that child really have cystic fibrosis? Lancet. 1978 Aug 19;2(8086):415–417. doi: 10.1016/s0140-6736(78)91878-0. [DOI] [PubMed] [Google Scholar]
- Wilkinson R. Polyethylene glycol 4000 as a continuously administered non-absorbable faecal marker for metabolic balance studies in human subjects. Gut. 1971 Aug;12(8):654–660. doi: 10.1136/gut.12.8.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolf R. O., Taussig L. M., Ross M. E., Wood R. E. Quantitative evaluation of serum pancreatic isoamylases in cystic fibrosis. J Lab Clin Med. 1976 Jan;87(1):164–168. [PubMed] [Google Scholar]