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. 1982 May;35(5):555–560. doi: 10.1136/jcp.35.5.555

A transport method for swab specimens submitted for aerobic and anaerobic bacteriology.

M Alfa, A Lee
PMCID: PMC497716  PMID: 7085900

Abstract

The need for separate swab transport methods for aerobes and anaerobes may result in inadequate transport of specimens for anaerobic bacteriology. Most microbiology laboratories in Australia rely on Stuart's transport medium to protect anaerobic bacteria. This paper presents a new, simple transport medium (Transport Deep) suitable for sue with aerobes and anaerobes. Comparative evaluations demonstrate that Transport Deep is as good as Stuart's medium for the maintenance of fastidious bacteria and is far superior for the protection of even extremely oxygen-sensitive anaerobes. This medium has been used successfully in a large Sydney hospital for more than a year. It is proposed that Transport Deep be used on a routine basis for all swab specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arank A., Syed S. A., Kenney E. B., Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. doi: 10.1128/am.17.4.568-576.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bartlett J. G., Sullivan-Sigler N., Louie T. J., Gorbach S. L. Anaerobes survive in clinical specimens despite delayed processing. J Clin Microbiol. 1976 Feb;3(2):133–136. doi: 10.1128/jcm.3.2.133-136.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartlett R. C., Tetreault J., Evers J., Officer J., Derench J. Quality assurance of gram-stained direct smears. Am J Clin Pathol. 1979 Dec;72(6):984–989. doi: 10.1093/ajcp/72.6.984. [DOI] [PubMed] [Google Scholar]
  4. Brewer J. H., Allgeier D. L. Safe Self-contained Carbon Dioxide-Hydrogen Anaerobic System. Appl Microbiol. 1966 Nov;14(6):985–988. doi: 10.1128/am.14.6.985-988.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dickman M. D., Chappelka A. R., Aff C., Gerhard J., Orcutt R. P. Compact anaerobic glove box for hospitals and research laboratories. J Clin Microbiol. 1979 Feb;9(2):294–296. doi: 10.1128/jcm.9.2.294-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fulghum R. S. Mobile anaerobe laboratory. Appl Microbiol. 1971 Apr;21(4):769–770. doi: 10.1128/am.21.4.769-770.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hill G. B. Effects of storage in an anaerobic transport system on bacteria in known polymicrobial mixtures and in clinical specimens. J Clin Microbiol. 1978 Dec;8(6):680–688. doi: 10.1128/jcm.8.6.680-688.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holland J. W., Hill E. O., Altemeier W. A. Numbers and types of anaerobic bacteria isolated from clinical specimens since 1960. J Clin Microbiol. 1977 Jan;5(1):20–25. doi: 10.1128/jcm.5.1.20-25.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaplan S., Atherly A. G., Barrett A. Synthesis of stable RNA in stringent Escherichia coli cells in the absence of charged transfer RNA. Proc Natl Acad Sci U S A. 1973 Mar;70(3):689–692. doi: 10.1073/pnas.70.3.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Killgore G. E., Starr S. E., Del Bene V. E., Whaley D. N., Dowell V. R., Jr Comparison of three anaerobic systems for the isolation of anaerobic bacteria from clinical specimens. Am J Clin Pathol. 1973 Apr;59(4):552–559. doi: 10.1093/ajcp/59.4.552. [DOI] [PubMed] [Google Scholar]
  11. McMinn M. T., Crawford J. J. Recovery of anaerobic microorganisms from clinical specimens in prereduced media versus recovery by routine clinical laboratory methods. Appl Microbiol. 1970 Feb;19(2):207–213. doi: 10.1128/am.19.2.207-213.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mena E., Thompson F. S., Armfield A. Y., Dowell V. R., Jr, Reinhardt D. J. Evaluation of Port-A-Cul transport system for protection of anaerobic bacteria. J Clin Microbiol. 1978 Jul;8(1):28–35. doi: 10.1128/jcm.8.1.28-35.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moore W. E., Cato E. P., Holdeman L. V. Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections. J Infect Dis. 1969 Jun;119(6):641–649. doi: 10.1093/infdis/119.6.641. [DOI] [PubMed] [Google Scholar]
  14. Mossel D. A., Beerens H. Studies on the inhibitory properties of sodium thioglycollate on the germination of wet spores of clostridia. J Hyg (Lond) 1968 Jun;66(2):269–272. doi: 10.1017/s0022172400041127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Peach S., Hayek L. The isolation of anaerobic bacteria from wound swabs. J Clin Pathol. 1974 Jul;27(7):578–582. doi: 10.1136/jcp.27.7.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wilkins T. D., Jimenez-Ulate F. Anaerobic specimen transport device. J Clin Microbiol. 1975 Nov;2(5):441–447. doi: 10.1128/jcm.2.5.441-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yrios J. W., Balish E., Helstad A., Field C., Inhorn S. Survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems. J Clin Microbiol. 1975 Feb;1(2):196–200. doi: 10.1128/jcm.1.2.196-200.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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