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. Author manuscript; available in PMC: 2016 Aug 8.
Published in final edited form as: Oncogene. 2012 Jan 30;31(48):5019–5028. doi: 10.1038/onc.2011.654

Figure 5. Heterozygosity for E2f1 is sufficient to block retinoblastoma.

Figure 5

(A) P0 and adult (P30–P60) retinal sections of the indicated genotypes were stained for mitotic cells (PH3, red) and nuclei (DAPI, blue), or H&E (lower panels). Apical mitoses (white arrows) represent normal progenitors while ectopic mitoses (red arrows) represent abnormally dividing differentiating neurons that are abundant in the Rb/p107 null retina, and reduced or virtually absent when one or two E2f1 alleles are removed, respectively. Scale bar, 50μm. The lens or tumors in adult H&E sections are indicated. For quantification of tumor frequency see Table 2. (B) Quantification of mitoses in indicated genotypes. Data are mean ± SD and asterisks indicate significant difference from WT (* P<0.05, ** P<0.01. Students t-test For simplicity “Rb−/−” represents αCre;Rbf/f in (A & B). NBL: neuroblastic layer, GCL: ganglion cell layer.