Abstract
A simple method for the quantitative assay of tissue plasminogen activator is described. Human veins and uterus obtained at operation are disintegrated in a membrane disintegrator at -70 degrees C and a known weight of the powder, suspended in buffered saline and thoroughly mixed. Assay of the dilutions of this homogenate on isotope-labelled fibrin clots gives straight line plots of log weight against log activity of the dilution and the sample activity is calculated from this. The method has been compared with the conventional histochemical technique. A highly significant correlation between the results of the two methods has been obtained (r = 0.79; p less than 0.001).
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ASTRUP T., ALBRECHTSEN O. K. Estimation of the plasminogen activator and the trypsin inhibitor in animal and human tissues. Scand J Clin Lab Invest. 1957;9(3):233–243. doi: 10.3109/00365515709079963. [DOI] [PubMed] [Google Scholar]
- Astedt B. Low fibrinolytic activity of veins during treatment with ethinylo estradiol. Acta Obstet Gynecol Scand. 1971;50(3):279–283. doi: 10.3109/00016347109157324. [DOI] [PubMed] [Google Scholar]
- Browse N. L., Gray L., Jarrett P. E., Morland M. Blood and vein-wall fibrinolytic activity in health and vascular disease. Br Med J. 1977 Feb 19;1(6059):478–481. doi: 10.1136/bmj.1.6059.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isacson S., Nilsson I. M. Defective fibrinolysis in blood and vein walls in recurrent "idiopathic" venous thrombosis. Acta Chir Scand. 1972;138(4):313–319. [PubMed] [Google Scholar]
- Johnson R. H., Mansfield A. O. A new method for the detection of the plasminogen activator content of vein walls. Acta Haematol. 1978;60(4):243–249. doi: 10.1159/000207720. [DOI] [PubMed] [Google Scholar]
- KWAAN H. C., ASTRUP T. LOCALIZATION OF FIBRINOLYTIC ACTIVITY IN THE EYE. Arch Pathol. 1963 Dec;76:595–601. [PubMed] [Google Scholar]
- Kwaan H. C., Astrup T. Demonstration of cellular fibrinolytic activity by the histochemical fibrin slide technique. Lab Invest. 1967 Aug;17(2):140–145. [PubMed] [Google Scholar]
- Nicolaides A. N., Clark C. T., Thomas R. D., Lewis J. D. Fibrinolytic activator in the endothelium of the veins of the lower limb. Br J Surg. 1976 Nov;63(11):881–884. doi: 10.1002/bjs.1800631112. [DOI] [PubMed] [Google Scholar]
- Pandolfi M., Nilsson I. M., Robertson B., Isacson S. Fibrinolytic activity of human veins. Lancet. 1967 Jul 15;2(7507):127–128. doi: 10.1016/s0140-6736(67)92964-9. [DOI] [PubMed] [Google Scholar]
- TODD A. S. Fibrinolysis autographs. Nature. 1958 Feb 15;181(4607):495–496. doi: 10.1038/181495b0. [DOI] [PubMed] [Google Scholar]