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. 1982 Nov;35(11):1227–1231. doi: 10.1136/jcp.35.11.1227

Immunological localisation of beta-thromboglobulin and platelet factor 4 in human megakaryocytes and platelets.

K M McLaren, D S Pepper
PMCID: PMC497932  PMID: 6183294

Abstract

Beta-thromboglobulin (beta TG) and platelet factor 4 (PF4) have been localised in megakaryocytes and platelets using immunofluorescence and immunoperoxidase techniques. These studies support the concept of synthesis of the proteins by parent megakaryocytes. By immunoelectron microscopy both proteins have been visualised in the alpha granule of the platelet and megakaryocyte, supporting functional studies of the cytoplasmic localisation of these proteins. The light microscopic techniques may allow elucidation of the distribution and role of the megakaryocyte in the pulmonary circulation and, on a practical level, permit its identification and distinction from other multinucleate cells in extramedullary tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burns J. Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material. Histochemistry. 1975 Jun 5;43(3):291–294. doi: 10.1007/BF00499711. [DOI] [PubMed] [Google Scholar]
  2. Day H. J., Stormorken H., Holmsen H. Subcellular localization of platelet factor 3 and platelet factor 4. Scand J Haematol. 1973;10(4):254–260. doi: 10.1111/j.1600-0609.1973.tb00069.x. [DOI] [PubMed] [Google Scholar]
  3. Hiti-Harper J., Wohl H., Harper E. Platelet factor 4: an inhibitor of collagenase. Science. 1978 Mar 3;199(4332):991–992. doi: 10.1126/science.203038. [DOI] [PubMed] [Google Scholar]
  4. Johnson A. R., Erdös E. G. Metabolism of vasoactive peptides by human endothelial cells in culture. Angiotensin I converting enzyme (kininase II) and angiotensinase. J Clin Invest. 1977 Apr;59(4):684–695. doi: 10.1172/JCI108687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Levine S. P., Wohl H. Human platelet factor 4: Purification and characterization by affinity chromatography. Purification of human platelet factor 4. J Biol Chem. 1976 Jan 25;251(2):324–328. [PubMed] [Google Scholar]
  6. Ludlam C. A., Cash J. D. Studies on liberation of beta-thromboglobulin from human platelets in vitro. Br J Haematol. 1976 Jun;33(2):239–247. doi: 10.1111/j.1365-2141.1976.tb03535.x. [DOI] [PubMed] [Google Scholar]
  7. McLaren K. M., Holloway L., Pepper D. S. Human platelet factor 4 and tissue mast cells. Thromb Res. 1980 Jul 1;19(1-2):293–297. doi: 10.1016/0049-3848(80)90431-4. [DOI] [PubMed] [Google Scholar]
  8. Moncada S., Korbut R., Bunting S., Vane J. R. Prostacyclin is a circulating hormone. Nature. 1978 Jun 29;273(5665):767–768. doi: 10.1038/273767a0. [DOI] [PubMed] [Google Scholar]
  9. Pedersen N. T. Occurrence of megakaryocytes in various vessels and their retention in the pulmonary capillaries in man. Scand J Haematol. 1978 Nov;21(5):369–375. doi: 10.1111/j.1600-0609.1978.tb00381.x. [DOI] [PubMed] [Google Scholar]
  10. Piovella F., Ascari E., Sitar G. M., Malamani G. D., Cattaneo G., Magliulo E., Storti E. Immunofluorescent detection of factor VIII-related antigen in human platelets and megakaryoctes. Haemostasis. 1974;3(5-6):288–295. doi: 10.1159/000214067. [DOI] [PubMed] [Google Scholar]
  11. Rabellino E. M., Nachman R. L., Williams N., Winchester R. J., Ross G. D. Human megakaryocytes. I. Characterization of the membrane and cytoplasmic components of isolated marrow megakaryocytes. J Exp Med. 1979 Jun 1;149(6):1273–1287. doi: 10.1084/jem.149.6.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Walsh P. N., Biggs R. The role of platelets in intrinsic factor-Xa formation. Br J Haematol. 1972 Jun;22(6):743–760. doi: 10.1111/j.1365-2141.1972.tb05719.x. [DOI] [PubMed] [Google Scholar]
  13. Walsh P. N., Biggs R. The role of platelets in intrinsic factor-Xa formation. Br J Haematol. 1972 Jun;22(6):743–760. doi: 10.1111/j.1365-2141.1972.tb05719.x. [DOI] [PubMed] [Google Scholar]
  14. Witte L. D., Kaplan K. L., Nossel H. L., Lages B. A., Weiss H. J., Goodman D. S. Studies of the release from human platelets of the growth factor for cultured human arterial smooth muscle cells. Circ Res. 1978 Mar;42(3):402–409. doi: 10.1161/01.res.42.3.402. [DOI] [PubMed] [Google Scholar]

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