Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1982 Nov;35(11):1253–1267. doi: 10.1136/jcp.35.11.1253

Use of monoclonal antibodies for the histopathological diagnosis of human malignancy

KC Gatter *, Z Abdulaziz *, P Beverley †, JRF Corvalan , C Ford ‡, EB Lane §, M Mota *, JRG Nash *, K Pulford *, H Stein ∥, J Taylor-Papadimitriou §, C Woodhouse ‡, DY Mason *
PMCID: PMC497939  PMID: 6183295

Abstract

This paper describes the use of a panel of seven monoclonal antibodies (selected so as to include reagents reactive with both epithelial and lymphoid cells) for distinguishing between anaplastic carcinoma and high grade lymphoma. Details are given of the immunohistological reactions of these antibodies against a wide range of both normal and malignant tissues and of a number of practical instances in which use of the antibody panel enabled a diagnosis to be made when routine histological examination had been inconclusive.

Full text

PDF
1253

Images in this article

1262Image
on p.1262
1256Image
on p.1256
1257Image
on p.1257
1257Image
on p.1257
1258Image
on p.1258
1259Image
on p.1259
1261Image
on p.1261
1263Image
on p.1263

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arklie J., Taylor-Papadimitrious J., Bodmer W., Egan M., Millis R. Differentiation antigens expressed by epithelial cells in the lactating breast are also detectable in breast cancers. Int J Cancer. 1981 Jul 15;28(1):23–29. doi: 10.1002/ijc.2910280105. [DOI] [PubMed] [Google Scholar]
  2. Banks-Schlegel S., Green H. Involucrin synthesis and tissue assembly by keratinocytes in natural and cultured human epithelia. J Cell Biol. 1981 Sep;90(3):732–737. doi: 10.1083/jcb.90.3.732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Battifora H., Sun T. T., Bahu R. M., Rao S. The use of antikeratin antiserum as a diagnostic tool: thymoma versus lymphoma. Hum Pathol. 1980 Nov;11(6):635–641. doi: 10.1016/s0046-8177(80)80074-8. [DOI] [PubMed] [Google Scholar]
  4. Forsum U., Klareskog L., Peterson P. A. Distribution of Ia-antigen-like molecules on non-lymphoid tissues. Scand J Immunol. 1979;9(4):343–349. doi: 10.1111/j.1365-3083.1979.tb03172.x. [DOI] [PubMed] [Google Scholar]
  5. Franke W. W., Appelhans B., Schmid E., Freudenstein C., Osborn M., Weber K. Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation. 1979;15(1):7–25. doi: 10.1111/j.1432-0436.1979.tb01030.x. [DOI] [PubMed] [Google Scholar]
  6. Franke W. W., Denk H., Kalt R., Schmid E. Biochemical and immunological identification of cytokeratin proteins present in hepatocytes of mammalian liver tissue. Exp Cell Res. 1981 Feb;131(2):299–318. doi: 10.1016/0014-4827(81)90234-2. [DOI] [PubMed] [Google Scholar]
  7. Franke W. W., Weber K., Osborn M., Schmid E., Freudenstein C. Antibody to prekeratin. Decoration of tonofilament like arrays in various cells of epithelial character. Exp Cell Res. 1978 Oct 15;116(2):429–445. doi: 10.1016/0014-4827(78)90466-4. [DOI] [PubMed] [Google Scholar]
  8. Goldenberg D. M., Sharkey R. M., Primus F. J. Carcinoembryonic antigen in histopathology: immunoperoxidase staining of conventional tissue sections. J Natl Cancer Inst. 1976 Jul;57(1):11–22. doi: 10.1093/jnci/57.1.11. [DOI] [PubMed] [Google Scholar]
  9. Halper J. P., Knowles D. M., 2nd, Wang C. Y. Ia antigen expression by human malignant lymphomas: correlation with conventional lymphoid markers. Blood. 1980 Mar;55(3):373–382. [PubMed] [Google Scholar]
  10. Hart D. N., Fabre J. W. Demonstration and characterization of Ia-positive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain. J Exp Med. 1981 Aug 1;154(2):347–361. doi: 10.1084/jem.154.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heyderman E., Neville A. M. A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products. J Clin Pathol. 1977 Feb;30(2):138–140. doi: 10.1136/jcp.30.2.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Janossy G., Thomas J. A., Pizzolo G., Granger S. M., McLaughlin J., Habeshaw J. A., Stansfeld A. G., Sloane J. Immuno-histological diagnosis of lymphoproliferative diseases by selected combinations of antisera and monoclonal antibodies. Br J Cancer. 1980 Aug;42(2):224–242. doi: 10.1038/bjc.1980.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kadin M. E. Ia-like (HLA-DR) antigens in the diagnosis of lymphoma and undifferentiated tumors. Arch Pathol Lab Med. 1980 Oct;104(10):503–508. [PubMed] [Google Scholar]
  14. Klareskog L., Forsum U., Peterson P. A. Hormonal regulation of the expression of Ia antigens on mammary gland epithelium. Eur J Immunol. 1980 Dec;10(12):958–963. doi: 10.1002/eji.1830101212. [DOI] [PubMed] [Google Scholar]
  15. Lampert I. A., Suitters A. J., Chisholm P. M. Expression of Ia antigen on epidermal keratinocytes in graft-versus-host disease. Nature. 1981 Sep 10;293(5828):149–150. doi: 10.1038/293149a0. [DOI] [PubMed] [Google Scholar]
  16. Löning T., Staquet M. J., Thivolet J., Seifert G. Keratin polypeptides distribution in normal and diseased human epidermis and oral mucosa. Immunohistochemical study on unaltered epithelium and inflammatory, premalignant and malignant lesions. Virchows Arch A Pathol Anat Histol. 1980;388(3):273–288. doi: 10.1007/BF00430859. [DOI] [PubMed] [Google Scholar]
  17. Mason D. W., Dallman M., Barclay A. N. Graft-versus-host disease induces expression of Ia antigen in rat epidermal cells and gut epithelium. Nature. 1981 Sep 10;293(5828):150–151. doi: 10.1038/293150a0. [DOI] [PubMed] [Google Scholar]
  18. Matoltsy A. G. Desmosomes, filaments, and keratohyaline granules: their role in the stabilization and keratinization of the epidermis. J Invest Dermatol. 1975 Jul;65(1):127–142. doi: 10.1111/1523-1747.ep12598093. [DOI] [PubMed] [Google Scholar]
  19. Natali P. G., De Martino C., Quaranta V., Bigotti A., Pellegrino M. A., Ferrone S. Changes in Ia-like antigen expression on malignant human cells. Immunogenetics. 1981;12(3-4):409–413. doi: 10.1007/BF01561680. [DOI] [PubMed] [Google Scholar]
  20. Natali P. G., De Martino C., Quaranta V., Nicotra M. R., Frezza F., Pellegrino M. A., Ferrone S. Expression of Ia-like antigens in normal human nonlymphoid tissues. Transplantation. 1981 Jan;31(1):75–78. doi: 10.1097/00007890-198101000-00017. [DOI] [PubMed] [Google Scholar]
  21. Pizzolo G., Sloane J., Beverley P., Thomas J. A., Bradstock K. F., Mattingly S., Janossy G. Differential diagnosis of malignant lymphoma and nonlymphoid tumors using monoclonal anti-leucocyte antibody. Cancer. 1980 Dec 15;46(12):2640–2647. doi: 10.1002/1097-0142(19801215)46:12<2640::aid-cncr2820461218>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  22. Sloane J. P., Ormerod M. G. Distribution of epithelial membrane antigen in normal and neoplastic tissues and it value in diagnostic tumor pathology. Cancer. 1981 Apr 1;47(7):1786–1795. doi: 10.1002/1097-0142(19810401)47:7<1786::aid-cncr2820470711>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  23. Steinert P. M., Cantieri J. S., Teller D. C., Lonsdale-Eccles J. D., Dale B. A. Characterization of a class of cationic proteins that specifically interact with intermediate filaments. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4097–4101. doi: 10.1073/pnas.78.7.4097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Steinman R. M. Dendritic cells. Transplantation. 1981 Mar;31(3):151–155. [PubMed] [Google Scholar]
  25. Sun T. T., Green H. Keratin filaments of cultured human epidermal cells. Formation of intermolecular disulfide bonds during terminal differentiation. J Biol Chem. 1978 Mar 25;253(6):2053–2060. [PubMed] [Google Scholar]
  26. Sun T. T., Shih C., Green H. Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2813–2817. doi: 10.1073/pnas.76.6.2813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Taylor-Papadimitriou J., Peterson J. A., Arklie J., Burchell J., Ceriani R. L., Bodmer W. F. Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer. 1981 Jul 15;28(1):17–21. doi: 10.1002/ijc.2910280104. [DOI] [PubMed] [Google Scholar]
  28. Walker R. A. Demonstration of carcinoembryonic antigen in human breast carcinomas by the immunoperoxidase technique. J Clin Pathol. 1980 Apr;33(4):356–360. doi: 10.1136/jcp.33.4.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Warnke R., Miller R., Grogan T., Pederson M., Dilley J., Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma. N Engl J Med. 1980 Aug 7;303(6):293–300. doi: 10.1056/NEJM198008073030601. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES