Table 1.
Phage resistance mutations in Pst DC3000
| Bacterial line | Mutation | Position | Gene | Selection experiment | Mutation effect |
|---|---|---|---|---|---|
| FRS | C → T | 1184566 | rfbA | 1 | Methionine → Isoleucine |
| NOI | C → T | 1184566 | rfbA | 1 | Methionine → Isoleucine |
| SNK | C → T | 1184566 | rfbA | 1 | Methionine → Isoleucine |
| SHL | C → T | 1185429 | rfbD | 1 | Tryptophan → Stop codon |
| WIL | C → T | 1185429 | rfbD | 1 | Tryptophan → Stop codon |
| REC | CAGC insertion | 47222 | No predicted function | 1 | Frameshift |
| NOI | T → G | 1184510 | rfbA | 2 | Histidine → Proline |
Each three‐letter code refers to a phage strain. Selection Experiment 1 was the generation of the phage resistant lines used in the assays, while Experiment 2 was to test for convergent molecular evolution.