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. 2016 Apr 25;35(45):5860–5871. doi: 10.1038/onc.2016.121

Figure 7.

Figure 7

NCOA3 regulates induction of XBP1 upon oestrogen signalling and is required for XBP1-mediated antioestrogen resistance. (a) pTRIPZshNCOA3-MCF7 cells were treated with (10 nm) oestrogen in the absence and presence of (500 ng/ml) of doxycycline for indicated time points. Line graphs show the absorbance in cells at the indicated time points after the E2 treatment. Error bars represent mean±s.d. from three independent experiments performed in triplicate. (b) pTRIPZshNCOA3-MCF7 cells were treated with (10 nm) oestrogen in the absence and presence of (500 ng/ml) of doxycycline for indicated time points. The expression level of spliced XBP1 (XBP1-S), GREB1 and NCOA3 was quantified by real-time RT–PCR, normalizing against RPLP0. Error bars represent mean±s.d. from three independent experiments performed in triplicate. (c) pTRIPZshNCOA3-MCF7 cells transfected with control or XBP1-S plasmid and were treated with (1 μm) fulvestrant in the absence and presence of (500 ng/ml) of doxycycline for 48 h. MTS cell proliferation assay was performed to assess the changes in cell density. *P<0.05, two-tailed unpaired t-test compared with untreated cells; **P<0.05, two-tailed unpaired t-test comparing respective time points. NS, not significant.