Skip to main content
. 2016 Jun 22;203(4):1613–1628. doi: 10.1534/genetics.116.191783

Figure 6.

Figure 6

Donor and target inserted at the same loci do not necessarily lead to high conversion frequencies. (A) An attB-QF2G4H donor insert at the attP2 site is used to HACK Janelia GMR-GAL4 lines inserted at attP2. (B) Examples of QF2 lines that were HACKed from widely used attP2-GMR-GAL4 lines. Colabeling experiments demonstrate expression patterns of QF2G4H recapitulate those of the original attP2-GMR-GAL4 insertion. Bar, 100 µm (brains, left panel), 20 µm (inset magnification). (C) The conversion rate of attP2-GMR-GAL4 lines using attP2-QF2G4H or nearby QF2G4H lines. The attP2-QF2G4H donors were either in forward (black arrow, as generated by pBPGUw-HACK_G4 > QF2) or reverse (red arrow, as generated by pHACK_G4 > QF2) orientations. Both exhibited significantly higher success rates than three nearby donors (ANOVA, P < 0.001). The conversion rate mediated by attP2-QF2G4H in the same orientation as attP2-GMR-GAL4 was significantly higher than the conversion rate mediated by attP2-QF2G4H inserted in the opposite orientation (P = 4.7 × 10−5, Student’s t-test).