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. 1983 Jun;36(6):697–700. doi: 10.1136/jcp.36.6.697

A fluorimetric method for red blood cell sorbitol dehydrogenase activity.

G Vaca, P Zúñiga, C Medina, R Alonso, G González-Quiroga, R I Ortíz-De-Luna, J M Cantú
PMCID: PMC498353  PMID: 6853734

Abstract

A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the present method offers several advantages: it is more rapid, simple and inexpensive. It should be useful to screen for sorbitol dehydrogenase deficiency in large numbers of individuals, particularly patients with diabetes or cataracts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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