Abstract
A discontinuous gradient of Percoll was used to remove RBCs from polymorphonuclear leucocyte (PMN) preparations. The resulting red blood cell-free preparation was used to investigate the effect of ammonium chloride on the iodination response of human PMN. Treatment of PMN with ammonium chloride for five minutes at room temperature resulted in a statistically significant increase in the iodination response of both resting and stimulated PMN.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aguado M. T., Pujol N., Rubiol E., Tura M., Celada A. Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran. J Immunol Methods. 1980;32(1):41–50. doi: 10.1016/0022-1759(80)90115-5. [DOI] [PubMed] [Google Scholar]
- Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
- Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
- Dewar C. An improved method for isolation of granulocytes from peripheral blood. J Immunol Methods. 1978;20:301–310. doi: 10.1016/0022-1759(78)90265-x. [DOI] [PubMed] [Google Scholar]
- Hart P. D. Macrophage antimicrobial activity: evidence for participation by lysosomes in the killing of Saccharomyces cerevisiae by normal resident macrophages. Infect Immun. 1981 Feb;31(2):828–830. doi: 10.1128/iai.31.2.828-830.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shortman K., Williams N., Adams P. The separation of different cell classes from lymphoid organs. V. Simple procedures for the removal of cell debris. Damaged cells and erythroid cells from lymphoid cell suspensions. J Immunol Methods. 1972 May;1(3):273–287. doi: 10.1016/0022-1759(72)90005-1. [DOI] [PubMed] [Google Scholar]
- Simchowitz L., Spilberg I. Chemotactic factor-induced generation of superoxide radicals by human neutrophils: evidence for the role of sodium. J Immunol. 1979 Nov;123(5):2428–2435. [PubMed] [Google Scholar]