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. 1983 Jul;36(7):811–815. doi: 10.1136/jcp.36.7.811

Release of beta-thromboglobulin during the preparation, in vitro storage and cryopreservation of platelet concentrates.

M A Taylor
PMCID: PMC498394  PMID: 6190844

Abstract

Beta-thromboglobulin is a platelet specific protein which is released from platelets during the platelet release reaction. The amount of beta-thromboglobulin released during various conditions of in vitro storage, including cryopreservation, was compared. The results suggest the measurement of beta-thromboglobulin is unlikely to be of use as a quality control assay for monitoring the in vitro viability of stored platelets.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boughton B. J., Allington M. J., King A. Platelet and plasma beta thromboglobulin in myeloproliferative syndromes and secondary thrombocytosis. Br J Haematol. 1978 Sep;40(1):125–132. doi: 10.1111/j.1365-2141.1978.tb03646.x. [DOI] [PubMed] [Google Scholar]
  2. Dawes J., Smith R. C., Pepper D. S. The release, distribution, and clearance of human beta-thromboglobulin and platelet factor 4. Thromb Res. 1978 May;12(5):851–861. doi: 10.1016/0049-3848(78)90279-7. [DOI] [PubMed] [Google Scholar]
  3. Eriksson L., Högman C. F., Busch C. Lack of conformity in the behaviour of platelets during normal storage conditions at 22 degrees C. Vox Sang. 1981 Feb;40(2):65–70. doi: 10.1111/j.1423-0410.1981.tb00671.x. [DOI] [PubMed] [Google Scholar]
  4. Filip D. J., Aster R. H. Relative hemostatic effectiveness of human platelets stored at 4 degrees and 22 degrees C. J Lab Clin Med. 1978 Apr;91(4):618–624. [PubMed] [Google Scholar]
  5. Handin R. I., Valeri C. R. Improved viability of previously frozen platelets. Blood. 1972 Oct;40(4):509–513. [PubMed] [Google Scholar]
  6. Kaplan K. L., Nossel H. L., Drillings M., Lesznik G. Radioimmunoassay of platelet factor 4 and beta-thromboglobulin: development and application to studies of platelet release in relation to fibrinopeptide A generation. Br J Haematol. 1978 May;39(1):129–146. doi: 10.1111/j.1365-2141.1978.tb07135.x. [DOI] [PubMed] [Google Scholar]
  7. Kattlove H. E. Platelet preservation--what temperature? A rationale for strategy. Transfusion. 1974 Jul-Aug;14(4):328–330. doi: 10.1111/j.1537-2995.1974.tb04540.x. [DOI] [PubMed] [Google Scholar]
  8. Kim B. K., Tanoue K., Baldini M. G. Storage of human platelets by freezing. Vox Sang. 1976;30(6):401–411. doi: 10.1111/j.1423-0410.1976.tb02845.x. [DOI] [PubMed] [Google Scholar]
  9. Moore S., Pepper D. S., Cash J. D. The isolation and characterisation of a platelet-specific beta-globulin (beta-thromboglobulin) and the detection of antiurokinase and antiplasmin released from thrombin-aggregated washed human platelets. Biochim Biophys Acta. 1975 Feb 27;379(2):360–369. doi: 10.1016/0005-2795(75)90143-9. [DOI] [PubMed] [Google Scholar]
  10. Mourad N. A simple method for obtaining platelet concentrates free of aggregates. Transfusion. 1968 Jan-Feb;8(1):48–48. doi: 10.1111/j.1537-2995.1968.tb02389.x. [DOI] [PubMed] [Google Scholar]
  11. Mourad N., Pert J. H. An enzymatic method to determine damage in human platelets. Proc Soc Exp Biol Med. 1967 Jun;125(2):643–645. doi: 10.3181/00379727-125-32168. [DOI] [PubMed] [Google Scholar]
  12. Murphy S., Sayar S. N., Abdou N. L., Gardner F. H. Platelet preservation by freezing. Use of dimethylsulfoxide as cryoprotective agent. Transfusion. 1974 Mar-Apr;14(2):139–144. doi: 10.1111/j.1537-2995.1974.tb04505.x. [DOI] [PubMed] [Google Scholar]
  13. Niewiarowski S., Walz D. A., James P., Rucinski B., Kueppers F. Identification and separation of secreted platelet proteins by isoelectric focusing. Evidence that low-affinity platelet factor 4 is converted to beta-thromboglobulin by limited proteolysis. Blood. 1980 Mar;55(3):453–456. [PubMed] [Google Scholar]
  14. Packham M. A., Mustard J. F. In vitro methods and the study of platelet mechanisms. Vox Sang. 1981;40 (Suppl 1):22–35. [PubMed] [Google Scholar]
  15. Pareti F. I., Dawes J., Franchi F., Mannucci P. M., Pepper D. S. Content and release of beta-thromboglobulin and platelet factor 4 in patients with qualitative platelet defects. Thromb Res. 1979;16(3-4):537–542. doi: 10.1016/0049-3848(79)90100-2. [DOI] [PubMed] [Google Scholar]
  16. Schiffer C. A., Aisner J., Wiernik P. H. Clinical experience with transfusion of cryopreserved platelets. Br J Haematol. 1976 Nov;34(3):377–385. doi: 10.1111/j.1365-2141.1976.tb03584.x. [DOI] [PubMed] [Google Scholar]
  17. Slichter S. J., Harker L. A. Preparation and storage of platelet concentrates. I. Factors influencing the harvest of viable platelets from whole blood. Br J Haematol. 1976 Nov;34(3):395–402. doi: 10.1111/j.1365-2141.1976.tb03586.x. [DOI] [PubMed] [Google Scholar]
  18. Slichter S. J., Harker L. A. Preparation and storage of platelet concentrates. II. Storage variables influencing platelet viability and function. Br J Haematol. 1976 Nov;34(3):403–419. doi: 10.1111/j.1365-2141.1976.tb03587.x. [DOI] [PubMed] [Google Scholar]
  19. Snyder E. L., Hezzey A., Katz A. J., Bock J. Occurrence of the release reaction during preparation and storage of platelet concentrates. Vox Sang. 1981 Sep;41(3):172–177. doi: 10.1111/j.1423-0410.1981.tb01032.x. [DOI] [PubMed] [Google Scholar]
  20. Tandy N. P., Taylor M. A. Platelet concentrates for transfusion: control of production and storage. Med Lab Sci. 1980 Mar;37(2):127–136. [PubMed] [Google Scholar]
  21. Weiss H. J., Witte L. D., Kaplan K. L., Lages B. A., Chernoff A., Nossel H. L., Goodman D. S., Baumgartner H. R. Heterogeneity in storage pool deficiency: studies on granule-bound substances in 18 patients including variants deficient in alpha-granules, platelet factor 4, beta-thromboglobulin, and platelet-derived growth factor. Blood. 1979 Dec;54(6):1296–1319. [PubMed] [Google Scholar]
  22. Zahavi J., Kakkar V. V. beta-Thromboglobulin--a specific marker of in-vivo platelet release reaction. Thromb Haemost. 1980 Aug 29;44(1):23–29. [PubMed] [Google Scholar]

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