Abstract
One hundred and seventeen specimens of cerebrospinal fluid from 94 patients were examined for the presence of pneumococcal and Haemophilus influenzae type b antigens using counterimmunoelectrophoresis and coagglutination tests. The coagglutination method using Phadebact reagents was as sensitive as counterimmunoelectrophoresis, but culture was a more sensitive diagnostic procedure than either test. A meningococcus coagglutination reagent, included in a prototype meningitis diagnostic kit, was also found to be as sensitive as counterimmunoelectrophoresis when tested on culture-positive cerebrospinal fluid specimens. Coagglutination tests for the detection of bacterial antigen are useful supportive tests when used in conjunction with direct microscopy and culture for bacterial pathogens.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Coonrod J. D., Rytel M. W. Determination of aetiology of bacterial meningitis by counter-immunoelectrophoresis. Lancet. 1972 May 27;1(7761):1154–1157. doi: 10.1016/s0140-6736(72)91376-1. [DOI] [PubMed] [Google Scholar]
- Curtis G. D., Slack M. P., Tompkins D. S. Cerebrospinal fluid lactate and the diagnosis of meningitis. J Infect. 1981 Jun;3(2):159–165. doi: 10.1016/s0163-4453(81)91336-0. [DOI] [PubMed] [Google Scholar]
- Feldman W. E. Effect of prior antibiotic therapy on concentrations of bacteria in CSF. Am J Dis Child. 1978 Jul;132(7):672–674. doi: 10.1001/archpedi.1978.02120320032006. [DOI] [PubMed] [Google Scholar]
- Fung J. C., Wicher K. Minimum number of bacteria needed for antigen detection by counterimmunoelectrophoresis: in vivo and in vitro studies. J Clin Microbiol. 1981 Apr;13(4):681–687. doi: 10.1128/jcm.13.4.681-687.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greenwood B. M., Whittle H. C., Dominic-Rajkovic O. Counter-current immunoelectrophoresis in the diagnosis of meningococcal infections. Lancet. 1971 Sep 4;2(7723):519–521. doi: 10.1016/s0140-6736(71)90439-9. [DOI] [PubMed] [Google Scholar]
- Kelsey M. C., Lipscomb A. P., Mowles J. M. Limulus amoebocyte lysate endotoxin test: an aid to the diagnosis in the septic neonate? J Infect. 1982 Jan;4(1):69–72. doi: 10.1016/s0163-4453(82)91104-5. [DOI] [PubMed] [Google Scholar]
- Moody G. J. Methodology and applications of countercurrent-immunoelectrophoresis in microbiology. Lab Pract. 1976 Sep;25(9):575–580. [PubMed] [Google Scholar]
- Olcén P. Serological methods for rapid diagnosis of haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae in cerebrospinal fluid: a comparison of Co-agglutination, immunofluorescence and immunoelectroosmophoresis. Scand J Infect Dis. 1978;10(4):283–289. doi: 10.3109/inf.1978.10.issue-4.05. [DOI] [PubMed] [Google Scholar]
- Peltola H. O. C-reactive protein for rapid monitoring of infections of the central nervous system. Lancet. 1982 May 1;1(8279):980–982. doi: 10.1016/s0140-6736(82)91989-4. [DOI] [PubMed] [Google Scholar]
- Shackelford P. G., Campbell J., Feigin R. D. Countercurrent immunoelectrophoresis in the evaluation of childhood infections. J Pediatr. 1974 Oct;85(4):478–481. doi: 10.1016/s0022-3476(74)80448-8. [DOI] [PubMed] [Google Scholar]
- Thirumoorthi M. C., Dajani A. S. Comparison of staphylococcal coagglutination, latex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection. J Clin Microbiol. 1979 Jan;9(1):28–32. doi: 10.1128/jcm.9.1.28-32.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tobin B. M., Jones D. M. Immunoelectroosmophoresis in the diagnosis of meningococcal infections. J Clin Pathol. 1972 Jul;25(7):583–585. doi: 10.1136/jcp.25.7.583. [DOI] [PMC free article] [PubMed] [Google Scholar]