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. 1983 Aug;36(8):929–934. doi: 10.1136/jcp.36.8.929

A miniaturised and simplified technique for typing and subtyping herpes simplex virus.

J M Darville
PMCID: PMC498424  PMID: 6308065

Abstract

A miniaturised, simplified and rapid method for typing and subtyping herpes simplex virus by restriction enzyme analysis of radioactively labelled DNA is described. This method requires far less label than those described previously, giving advantages in both cost and safety and therefore in its suitability for use in a routine laboratory. It produces results indistinguishable from those obtained by the parent method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamin D. R. Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method. Appl Microbiol. 1974 Oct;28(4):568–571. doi: 10.1128/am.28.4.568-571.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buchman T. G., Roizman B., Adams G., Stover B. H. Restriction endonuclease fingerprinting of herpes simplex virus DNA: a novel epidemiological tool applied to a nosocomial outbreak. J Infect Dis. 1978 Oct;138(4):488–498. doi: 10.1093/infdis/138.4.488. [DOI] [PubMed] [Google Scholar]
  3. Ejercito P. M., Kieff E. D., Roizman B. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968 May;2(3):357–364. doi: 10.1099/0022-1317-2-3-357. [DOI] [PubMed] [Google Scholar]
  4. Graham F. L., Smiley J., Russell W. C., Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977 Jul;36(1):59–74. doi: 10.1099/0022-1317-36-1-59. [DOI] [PubMed] [Google Scholar]
  5. Hill T. J., Field H. J., Blyth W. A. Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. J Gen Virol. 1975 Sep;28(3):341–353. doi: 10.1099/0022-1317-28-3-341. [DOI] [PubMed] [Google Scholar]
  6. Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Linnemann C. C., Jr, Buchman T. G., Light I. J., Ballard J. L. Transmission of herpes-simplex virus type 1 in a nursery for the newborn. Identification of viral isolates by D.N.A. "fingerprinting". Lancet. 1978 May 6;1(8071):964–966. doi: 10.1016/s0140-6736(78)90251-9. [DOI] [PubMed] [Google Scholar]
  8. Lonsdale D. M. A rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology. Lancet. 1979 Apr 21;1(8121):849–852. doi: 10.1016/s0140-6736(79)91265-0. [DOI] [PubMed] [Google Scholar]
  9. Maitland N. J., Smith I. W., Peutherer J. F., Robertson D. H., Jones K. W. Restriction endonuclease analysis of DNA from genital isolates of herpes simplex virus type 2. Infect Immun. 1982 Dec;38(3):834–842. doi: 10.1128/iai.38.3.834-842.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pauls F. P., Dowdle W. R. A serologic study of herpesvirus hominis strains by microneutralization tests. J Immunol. 1967 May;98(5):941–947. [PubMed] [Google Scholar]
  11. Pereira L., Cassai E., Honess R. W., Roizman B., Terni M., Nahmias A. Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology. Infect Immun. 1976 Jan;13(1):211–220. doi: 10.1128/iai.13.1.211-220.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peutherer J. F., Smith I. W., Robertson D. H. Genital infection with herpes simplex virus type I. J Infect. 1982 Jan;4(1):33–35. doi: 10.1016/s0163-4453(82)90930-6. [DOI] [PubMed] [Google Scholar]
  13. Schaeffer H. J., Beauchamp L., de Miranda P., Elion G. B., Bauer D. J., Collins P. 9-(2-hydroxyethoxymethyl) guanine activity against viruses of the herpes group. Nature. 1978 Apr 13;272(5654):583–585. doi: 10.1038/272583a0. [DOI] [PubMed] [Google Scholar]
  14. Schwartz J., Roizman B. Similarities and Differences in the Development of Laboratory Strains and Freshly Isolated Strains of Herpes Simplex Virus in HEp-2 Cells: Electron Microscopy. J Virol. 1969 Dec;4(6):879–889. doi: 10.1128/jvi.4.6.879-889.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Skare J., Summers W. P., Summers W. C. Structure and function of herpesvirus genomes. I. comparison of five HSV-1 and two HSV-2 strains by cleavage their DNA with eco R I restriction endonuclease. J Virol. 1975 Apr;15(4):726–732. doi: 10.1128/jvi.15.4.726-732.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith I. W., Maitland N. J., Peutherer J. F., Robertson D. H. Restriction enzyme analysis of herpesvirus-2 DNA. Lancet. 1981 Dec 19;2(8260-61):1424–1424. doi: 10.1016/s0140-6736(81)92840-3. [DOI] [PubMed] [Google Scholar]
  17. Tullo A. B., Shimeld C., Easty D. L., Darville J. M. Distribution of latent herpes simplex virus in human trigeminal ganglion. Lancet. 1983 Feb 12;1(8320):353–353. doi: 10.1016/s0140-6736(83)91652-5. [DOI] [PubMed] [Google Scholar]
  18. Wadell G., Sundell G., de Jong J. C. Characterization of candidate adenovirus 37 by SDS-polyacrylamide gel electrophoresis of virion polypeptides and DNA restriction site mapping. J Med Virol. 1981;7(2):119–125. doi: 10.1002/jmv.1890070205. [DOI] [PubMed] [Google Scholar]
  19. Whitley R., Lakeman A. D., Nahmias A., Roizman B. Dna restriction-enzyme analysis of herpes simplex virus isolates obtained from patients with encephalitis. N Engl J Med. 1982 Oct 21;307(17):1060–1062. doi: 10.1056/NEJM198210213071706. [DOI] [PubMed] [Google Scholar]

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