Abstract
In order to assess the practical value of immunocytochemical techniques in routine cytological diagnosis, a panel of three monoclonal antibodies (anti-CEA, Ca 1 and HMFG-2) were used to search for malignant cells in 53 samples of pleural or peritoneal fluid from 41 selected patients. All these patients suffered from malignant disease, but neoplastic cells had not been reported on conventional cytological examination. In 12 of the 41 cases immunocytochemical staining revealed previously unrecognised malignant cells. This represents an increase in diagnostic accuracy of approximately 20% and suggests that immunocytochemical labelling should be used routinely for the examination of cytologically negative samples from patients with suspected malignant disease.
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Selected References
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- Ashall F., Bramwell M. E., Harris H. A new marker for human cancer cells. 1 The Ca antigen and the Ca1 antibody. Lancet. 1982 Jul 3;2(8288):1–6. doi: 10.1016/s0140-6736(82)91150-3. [DOI] [PubMed] [Google Scholar]
- Burnett R. A., Deery A. R., Adamson M. R., Liddle C., Thomas M., Roberts G. H. Evaluation of Cal antibody in pleural biopsy material. Lancet. 1983 May 21;1(8334):1158–1158. doi: 10.1016/s0140-6736(83)92884-2. [DOI] [PubMed] [Google Scholar]
- Gatter K. C., Abdulaziz Z., Beverley P., Corvalan J. R., Ford C., Lane E. B., Mota M., Nash J. R., Pulford K., Stein H. Use of monoclonal antibodies for the histopathological diagnosis of human malignancy. J Clin Pathol. 1982 Nov;35(11):1253–1267. doi: 10.1136/jcp.35.11.1253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ghosh A. K., Spriggs A. I., Taylor-Papadimitriou J., Mason D. Y. Immunocytochemical staining of cells in pleural and peritoneal effusions with a panel of monoclonal antibodies. J Clin Pathol. 1983 Oct;36(10):1154–1164. doi: 10.1136/jcp.36.10.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McGee J. O., Woods J. C., Ashall F., Bramwell M. E., Harris H. A new marker for human cancer cells, 2 immunohistochemical detection of the Ca antigen in human tissues with the Ca1 antibody. Lancet. 1982 Jul 3;2(8288):7–10. doi: 10.1016/s0140-6736(82)91151-5. [DOI] [PubMed] [Google Scholar]
- Pallesen G., Jepsen F. L., Hastrup J., Ipsen A., Hvidberg N. Experience with the Oxford tumour marker (Ca1) in serous fluids. Lancet. 1983 Jun 11;1(8337):1326–1326. doi: 10.1016/s0140-6736(83)92430-3. [DOI] [PubMed] [Google Scholar]
- Sehested M., Ralfkjaer E., Rasmussen J. Immunoperoxidase demonstration of carcinoembryonic antigen in pleural and peritoneal effusions. Acta Cytol. 1983 Mar-Apr;27(2):124–127. [PubMed] [Google Scholar]
- To A., Dearnaley D. P., Ormerod M. G., Canti G., Coleman D. V. Epithelial Membrane Antigen. Its use in the cytodiagnosis of malignancy in serous effusions. Am J Clin Pathol. 1982 Aug;78(2):214–219. doi: 10.1093/ajcp/78.2.214. [DOI] [PubMed] [Google Scholar]
- Woods J. C., Spriggs A. I., Harris H., McGee J. O. A new marker for human cancer cells. 3. Immunocytochemical detection of malignant cells in serous fluids with the Ca1 antibody. Lancet. 1982 Sep 4;2(8297):512–514. doi: 10.1016/s0140-6736(82)90598-0. [DOI] [PubMed] [Google Scholar]