Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1983 Oct;36(10):1154–1164. doi: 10.1136/jcp.36.10.1154

Immunocytochemical staining of cells in pleural and peritoneal effusions with a panel of monoclonal antibodies.

A K Ghosh, A I Spriggs, J Taylor-Papadimitriou, D Y Mason
PMCID: PMC498494  PMID: 6194183

Abstract

A panel of seven monoclonal antibodies was applied to smears of cell deposit from 70 pleural and peritoneal fluids, using an immunoalkaline phosphatase (IAP) procedure. The cases were chosen to show typical cytological patterns, both benign and malignant, and in this way the diagnostic value of the method could be assessed. The antibodies used were 2D1 (anti-leucocyte), Ca 1, HMFG-2 (anti-milk fat globule membrane), LE61 and M73 (both anti-intermediate filament antibodies), anti-CEA, and K92 (anti-keratin). The anti-leucocyte antibody was found useful for distinguishing lymphoma from carcinoma. Anti-CEA gave positive reactions in 80% of carcinoma cases and was not seen to react with any other cell types. Ca 1 was positive with some cells in 95% of carcinoma cases, but mesothelial cells reacted with it in two cases. A strong reaction with the anti-milk fat globule membrane antibody was very constant in carcinoma but was also seen in mesothelial cells in 30% of benign effusions. The anti-keratin reacted with malignant cells in only a small proportion of cases. The antibodies against epithelial intermediate filaments reacted equally strongly with benign mesothelial cells and carcinoma cells, but gave negative reactions with lymphoma cells. It is concluded that a suitably chosen panel of monoclonal antibodies can be of great value in identifying neoplastic cells in serous effusions.

Full text

PDF
1154

Images in this article

1157Image
on p.1157
1159Image
on p.1159
1160Image
on p.1160
1160Image
on p.1160
1161Image
on p.1161
1161Image
on p.1161

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Epenetos A. A., Canti G., Taylor-Papadimitriou J., Curling M., Bodmer W. F. Use of two epithelium-specific monoclonal antibodies for diagnosis of malignancy in serous effusions. Lancet. 1982 Nov 6;2(8306):1004–1006. doi: 10.1016/s0140-6736(82)90047-2. [DOI] [PubMed] [Google Scholar]
  2. Gatter K. C., Abdulaziz Z., Beverley P., Corvalan J. R., Ford C., Lane E. B., Mota M., Nash J. R., Pulford K., Stein H. Use of monoclonal antibodies for the histopathological diagnosis of human malignancy. J Clin Pathol. 1982 Nov;35(11):1253–1267. doi: 10.1136/jcp.35.11.1253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lane E. B. Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization. J Cell Biol. 1982 Mar;92(3):665–673. doi: 10.1083/jcb.92.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mariani-Costantini R., Ménard S., Clemente C., Tagliabue E., Colnaghi M. I., Rilke F. Immunocytochemical identification of breast carcinoma cells in effusions using a monoclonal antibody. J Clin Pathol. 1982 Sep;35(9):1037–1037. doi: 10.1136/jcp.35.9.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. McGee J. O., Woods J. C., Ashall F., Bramwell M. E., Harris H. A new marker for human cancer cells, 2 immunohistochemical detection of the Ca antigen in human tissues with the Ca1 antibody. Lancet. 1982 Jul 3;2(8288):7–10. doi: 10.1016/s0140-6736(82)91151-5. [DOI] [PubMed] [Google Scholar]
  6. Nadji M. The potential value of immunoperoxidase techniques in diagnostic cytology. Acta Cytol. 1980 Sep-Oct;24(5):442–447. [PubMed] [Google Scholar]
  7. O'Brien M. J., Kirkham S. E., Burke B., Ormerod M., Saravis C. A., Gottlieb L. S., Neville A. M., Zamcheck N. CEA, ZGM and EMA localization in cells of pleural and peritoneal effusion: a preliminary study. Invest Cell Pathol. 1980 Jul-Sep;3(3):251–258. [PubMed] [Google Scholar]
  8. Pizzolo G., Sloane J., Beverley P., Thomas J. A., Bradstock K. F., Mattingly S., Janossy G. Differential diagnosis of malignant lymphoma and nonlymphoid tumors using monoclonal anti-leucocyte antibody. Cancer. 1980 Dec 15;46(12):2640–2647. doi: 10.1002/1097-0142(19801215)46:12<2640::aid-cncr2820461218>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  9. Singh G., Whiteside T. L., Dekker A. Immunodiagnosis of mesothelioma: use of antimesothelial cell serum in an indirect immunofluorescence assay. Cancer. 1979 Jun;43(6):2288–2296. doi: 10.1002/1097-0142(197906)43:6<2288::aid-cncr2820430619>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  10. Taylor-Papadimitriou J., Peterson J. A., Arklie J., Burchell J., Ceriani R. L., Bodmer W. F. Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer. 1981 Jul 15;28(1):17–21. doi: 10.1002/ijc.2910280104. [DOI] [PubMed] [Google Scholar]
  11. To A., Coleman D. V., Dearnaley D. P., Ormerod M. G., Steele K., Neville A. M. Use of antisera to epithelial membrane antigen for the cytodiagnosis of malignancy in serous effusions. J Clin Pathol. 1981 Dec;34(12):1326–1332. doi: 10.1136/jcp.34.12.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. To A., Dearnaley D. P., Ormerod M. G., Canti G., Coleman D. V. Epithelial Membrane Antigen. Its use in the cytodiagnosis of malignancy in serous effusions. Am J Clin Pathol. 1982 Aug;78(2):214–219. doi: 10.1093/ajcp/78.2.214. [DOI] [PubMed] [Google Scholar]
  13. Woods J. C., Spriggs A. I., Harris H., McGee J. O. A new marker for human cancer cells. 3. Immunocytochemical detection of malignant cells in serous fluids with the Ca1 antibody. Lancet. 1982 Sep 4;2(8297):512–514. doi: 10.1016/s0140-6736(82)90598-0. [DOI] [PubMed] [Google Scholar]
  14. Wu Y. J., Parker L. M., Binder N. E., Beckett M. A., Sinard J. H., Griffiths C. T., Rheinwald J. G. The mesothelial keratins: a new family of cytoskeletal proteins identified in cultured mesothelial cells and nonkeratinizing epithelia. Cell. 1982 Dec;31(3 Pt 2):693–703. doi: 10.1016/0092-8674(82)90324-5. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES