Abstract
A newly designed instrument is described which generates continuous records of the opacities of six bacterial cultures growing under strict anaerobic conditions. Additions (for example, of antibacterial agents) or withdrawal of culture (for example, for viable counting) can be made at any time without breach of anaerobiosis. Use of the instrument is illustrated by growth curves obtained from small inocula of two strict anaerobes, Bacteroides asaccharolyticus and Peptostreptococcus anaerobius and by the effects on the growth curve of Bacteroides fragilis of adding various concentrations of metronidazole at different times.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Eley A., Greenwood D. In vitro activity of ceftizoxime against Bacteroides fragilis: comparison with benzylpenicillin, cephalothin, and cefoxitin. Antimicrob Agents Chemother. 1981 Sep;20(3):332–335. doi: 10.1128/aac.20.3.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mackintosh I. P., O'Grady F., Greenwood D., Watson B. W., Crichton T. C., Piper R., Ferrer A. A twelve channel bacterial growth monitoring system. Biomed Eng. 1973 Dec;8(12):514–passim. [PubMed] [Google Scholar]
- Marchand A., Piaud J. J., Du Pasquier P. A high-precision and large-capacity laser biophotometer. Appl Microbiol. 1974 May;27(5):874–877. doi: 10.1128/am.27.5.874-877.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray P. R., Niles A. C. Adaptation of an automated microbiology system for the growth of anaerobes and performance of antimicrobial susceptibility tests. Am J Clin Pathol. 1982 Oct;78(4):457–461. doi: 10.1093/ajcp/78.4.457. [DOI] [PubMed] [Google Scholar]
- Murray P. R., Niles A. C. Inactivation of penicillins by Thiol broth. J Clin Microbiol. 1982 Nov;16(5):982–984. doi: 10.1128/jcm.16.5.982-984.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watson B. W., Gauci C. L., Blache L., O'Grady F. W. A simple turbidity cell for continuously monitoring the growth of bacteria. Phys Med Biol. 1969 Oct;14(4):555–558. doi: 10.1088/0031-9155/14/4/302. [DOI] [PubMed] [Google Scholar]
- Yourassowsky E., Van der Linden M. P., Lismont M. J., Labbé M., Crokaert F. The effect of beta-lactam antibiotics on the growth curves of Bacteroides fragilis, obtained with the MS-2 system. J Antimicrob Chemother. 1981 Sep;8(3):219–224. doi: 10.1093/jac/8.3.219. [DOI] [PubMed] [Google Scholar]