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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1983 Nov;36(11):1233–1236. doi: 10.1136/jcp.36.11.1233

Use of gas-liquid chromatography as a screening test for toxigenic Clostridium difficile in diarrhoeal stools.

F Pepersack, M Labbe, C Nonhoff, E Schoutens
PMCID: PMC498537  PMID: 6630574

Abstract

In order to determine if gas-liquid chromatography (GLC) on concentrated stool extracts could be substituted to cell culture assay for cytotoxicity, we prospectively studied 154 diarrhoeal stools submitted for detection of Clostridium difficile toxin. Isocaproic-positive samples were cultured on egg yolk agar supplemented with cycloserine, cefoxitin and fructose for isolation of C difficile, and on egg yolk agar plus kanamycin for isolation of other clostridium species. Of the 154 samples, 129 were GLC-negative (height of the isocaproic peak less than 1.2 cm) and were toxin-negative. Twenty-five stools yielded isocaproic acid; C difficile isolated from 13 of them, six of which were also toxin-positive. Four other isocaproic-positive samples yielded C bifermentans and C sordellii; all were toxin-negative. These results indicate that a negative GLC is an excellent screening test for excluding C difficile infection; positive results must be checked by toxin testing and culture since they are not necessarily associated with the presence of C difficile or its toxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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