Abstract
Untreated earlobe capillary blood samples from 5958 girls were screened for rubella antibodies by placing them directly onto haemolysis-in-gel (HIG) test plates. The method is in our experience quick, reliable and also acceptable to young girls. The method is convenient for testing for natural immunity before vaccination and in most but not all cases satisfactory as a test for seroconversion after vaccination. A comparison was made between naturally infected girls and a group of vaccinated girls. Vaccinated girls had lower values and most of the low or doubtful positives were in this group. Serum samples from 51 low positive girls were tested by the haemagglutination inhibition (HI) test and the serum HIG test and their results compared with their respective direct HIG values.
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Selected References
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- Banatvala J. E. Rubella vaccination: remaining problems. Br Med J (Clin Res Ed) 1982 May 1;284(6325):1285–1286. doi: 10.1136/bmj.284.6325.1285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buser F., Nicolas A., L'Etoile M. Vaccination with RA 27-3 rubella vaccine. Am J Dis Child. 1971 Jul;122(1):53–56. doi: 10.1001/archpedi.1971.02110010089015. [DOI] [PubMed] [Google Scholar]
- Einarsdóttir B., Rafnar B. Direct HIG test for rubella antibodies. J Med Virol. 1980;5(4):303–308. doi: 10.1002/1096-9071(1980)5:4<303::aid-jmv1890050406>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
- Grillner L., Strannegård O. Evaluation of the hemolysis-in-gel test for the screening of rubella immunity and the demonstration of recent infection. J Clin Microbiol. 1976 Feb;3(2):86–90. doi: 10.1128/jcm.3.2.86-90.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halonen P. E., Ryan J. M., Stewart J. A. Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK-21 cells. Proc Soc Exp Biol Med. 1967 May;125(1):162–167. doi: 10.3181/00379727-125-32038. [DOI] [PubMed] [Google Scholar]
- Ogra P. L., Kerr-Grant D., Umana G., Dzierba J., Weintraub D. Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus. N Engl J Med. 1971 Dec 9;285(24):1333–1339. doi: 10.1056/NEJM197112092852401. [DOI] [PubMed] [Google Scholar]
- Vaheri A., Vänänen P., Salonen E. M., Suni J. Rubella antibody determination from heparinised finger-tip blood by single radial haemolysis and enzyme immunoassay. J Clin Pathol. 1980 Sep;33(9):845–847. doi: 10.1136/jcp.33.9.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zippel C., Federmann G., Leidel J., Eggers H. J. Quantitativer Nachweis von Röteln-Virusantikörpern im Kapillarblut. Eine einfache, verlässliche und ökonomische Methode. MMW Munch Med Wochenschr. 1980 Jun 20;122(25):943–946. [PubMed] [Google Scholar]