Table 1.

Experimental output of MAP cells mL⁻¹ of milk obtained by IS900‐qPCR and f57‐qPCR after spiking negative milk samples with known amount of MAP

MAP strain	Input MAP cell concentration (f57‐qPCR)a	Input MAP cell concentration (culture)b	Experimental output of MAP cells by IS900 qPCRc			Experimental output of MAP cells by f57 qPCR d
			Meane	SD	Signal ratiof	Meane	SD	Signal ratiof
ATCC19698	1.0 × 104	6.5 × 103	1.3 × 103	2.7 × 102	10/10	1.8 × 103	3.1 × 102	10/10
	1.0 × 103	6.5 × 102	2.5 × 102	12.0 × 101	10/10	2.5 × 102	12.8 × 101	10/10
	1.0 × 102	6.5 × 101	2.7 × 101	2.1 × 101	10/10	3.1 × 101	2.0 × 101	7/10
	1.0 × 101	—	3.4 × 100	3.7 × 100	8/10	—	—	0/10
IZSLER76/13	0.7 × 104	2.3 × 102	2.4 × 103	5.3 × 102	10/10	1.9 × 103	2.2 × 102	10/10
	0.7 × 103	2.3 × 101	1.8 × 102	10.3 × 101	10/10	1.2 × 102	6.6 × 101	10/10
	0.7 × 102	—	1.5 × 101	7.9 × 100	10/10	1.8 × 101	1.5 × 101	6/10
	0.7 × 101	—	0.5 × 100	0.4 × 100	5/10	—	—	0/10

^aNumber of MAP cells mL⁻¹ in the pure cultures/suspensions used for the spiking of milk samples evaluated by f57 qPCR.

^bNumber of MAP cells mL⁻¹ in the pure cultures/suspensions used for the spiking of milk samples evaluated on culture in Herrold's Egg Yolk Agar with Mycobactin‐ANV plates in duplicate by seeding 100 μL and expressed as CFU mL⁻¹.

^cNumber of MAP cells mL⁻¹ recovered after DNA isolation by IS900 qPCR, the value was divided by 15 to obtain real number of MAP cells.

^dNumber of MAP cells mL⁻¹ recovered after DNA isolation by f57 qPCR.

^eThe mean values correspond to the absolute number of MAP cells mL⁻¹.

^fNumber of positive replicates/total number of replicates.