Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1984 Jan;37(1):37–44. doi: 10.1136/jcp.37.1.37

Immunohistochemical localisation of factor VIII-related antigen in Hodgkin's disease.

J Crocker, P J Smith
PMCID: PMC498615  PMID: 6423698

Abstract

A series of 45 lymph nodes involved by Hodgkin's disease have been examined by means of the unlabelled antibody peroxidase-antiperoxidase (PAP) method for the demonstration of factor VIII-related antigen (F VIII R Ag). In addition, five lymph nodes showing reactive follicular hyperplasia were studied. In the specimens of nodular sclerosing Hodgkin's disease, many blood vessels were present, as demonstrated by virtue of F VIII R Ag in endothelial cells. These vessels were present within and, especially, around the nodules in this subtype; in addition the fibrous trabeculae were densely vascular. High vascularity was also a feature of specimens of the cellular variant of nodular sclerosing Hodgkin's disease. However, the other three Rye subtypes of the disease showed a striking paucity of F VIII R Ag-positive blood vessels. The reactive nodes showed numerous vessels around and between follicles but the lymph sinuses were consistently negative. Mast cells were present in all specimens but were especially frequent within and adjacent to the sinuses in reactive specimens; they were strongly positive for the F VIII R Ag, their staining being abolished by pre-adsorption of the primary antiserum with factor VIII itself.

Full text

PDF
37

Images in this article

38Image
on p.38
39Image
on p.39
39Image
on p.39
40Image
on p.40
40Image
on p.40
41Image
on p.41
41Image
on p.41
42Image
on p.42
42Image
on p.42

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom A. L., Giddings J. C., Wilks C. J. Factor 8 on the vascular intima: possible importance in haemostasis and thrombosis. Nat New Biol. 1973 Feb 14;241(111):217–219. doi: 10.1038/newbio241217a0. [DOI] [PubMed] [Google Scholar]
  2. Burgdorf W. H., Mukai K., Rosai J. Immunohistochemical identification of factor VIII-related antigen in endothelial cells of cutaneous lesions of alleged vascular nature. Am J Clin Pathol. 1981 Feb;75(2):167–171. doi: 10.1093/ajcp/75.2.167. [DOI] [PubMed] [Google Scholar]
  3. Curran R. C., Gregory J., Jones E. L. The distribution of immunoglobulin and other plasma proteins in human reactive lymph nodes. J Pathol. 1982 Apr;136(4):307–332. doi: 10.1002/path.1711360406. [DOI] [PubMed] [Google Scholar]
  4. Curran R. C., Gregory J. The unmasking of antigens in paraffin sections of tissue by trypsin. Experientia. 1977 Oct 15;33(10):1400–1401. doi: 10.1007/BF01920206. [DOI] [PubMed] [Google Scholar]
  5. Freemont A. J., Jones C. J. Light microscopic, histochemical and ultrastructural studies of human lymph node paracortical venules. J Anat. 1983 Mar;136(Pt 2):349–362. [PMC free article] [PubMed] [Google Scholar]
  6. Guarda L. A., Ordonez N. G., Smith J. L., Jr, Hanssen G. Immunoperoxidase localization of factor VIII in angiosarcomas. Arch Pathol Lab Med. 1982 Oct;106(10):515–516. [PubMed] [Google Scholar]
  7. Hoyer L. W., De los Santos R. P., Hoyer J. R. Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy. J Clin Invest. 1973 Nov;52(11):2737–2744. doi: 10.1172/JCI107469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jaffe E. A. Endothelial cells and the biology of factor VIII. N Engl J Med. 1977 Feb 17;296(7):377–383. doi: 10.1056/NEJM197702172960707. [DOI] [PubMed] [Google Scholar]
  9. Jaffe E. A., Hoyer L. W., Nachman R. L. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest. 1973 Nov;52(11):2757–2764. doi: 10.1172/JCI107471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jaffe E. A. Synthesis of factor VIII antigen by cultured human endothelial cells. Ann N Y Acad Sci. 1975 Jan 20;240:62–69. doi: 10.1111/j.1749-6632.1975.tb53322.x. [DOI] [PubMed] [Google Scholar]
  11. Jeanneau C., Sultan Y. Localization of factor VIII/von Willebrand factor antigen by immunoelectron microscopy in human endothelial cells using Fab fragments coupled to peroxidase. J Histochem Cytochem. 1982 Nov;30(11):1091–1096. doi: 10.1177/30.11.6815260. [DOI] [PubMed] [Google Scholar]
  12. Kindblom L. G. Factor VIII related antigen and mast cells. Acta Pathol Microbiol Immunol Scand A. 1982 Nov;90(6):437–439. doi: 10.1111/j.1699-0463.1982.tb00119_90a.x. [DOI] [PubMed] [Google Scholar]
  13. McComb R. D., Jones T. R., Pizzo S. V., Bigner D. D. Specificity and sensitivity of immunohistochemical detection of factor VIII/von Willebrand factor antigen in formalin-fixed paraffin-embedded tissue. J Histochem Cytochem. 1982 Apr;30(4):371–377. doi: 10.1177/30.4.6801111. [DOI] [PubMed] [Google Scholar]
  14. McKee P. A., Andersen J. C., Switzer M. E. Molecular structural studies of human factor VIII. Ann N Y Acad Sci. 1975 Jan 20;240:8–33. doi: 10.1111/j.1749-6632.1975.tb53319.x. [DOI] [PubMed] [Google Scholar]
  15. Mukai K., Rosai J., Burgdorf W. H. Localization of factor VIII-related antigen in vascular endothelial cells using an immunoperoxidase method. Am J Surg Pathol. 1980 Jun;4(3):273–276. doi: 10.1097/00000478-198006000-00008. [DOI] [PubMed] [Google Scholar]
  16. Nanba K., Itagaki T., Iijima S. Enzyme histochemical investigations of human malignant lymphomas. Beitr Pathol. 1975;154(3):233–242. doi: 10.1016/s0005-8165(75)80032-1. [DOI] [PubMed] [Google Scholar]
  17. Nanba K., Jaffe E. S., Braylan R. C., Soban E. J., Berard C. W. Alkaline phosphatase-positive malignant lymphoma. A subtype of B-cell lymphomas. Am J Clin Pathol. 1977 Nov;68(5):535–542. doi: 10.1093/ajcp/68.5.535. [DOI] [PubMed] [Google Scholar]
  18. Order S. E., Hellman S. Pathogenesis of Hodgkin's disease. Lancet. 1972 Mar 11;1(7750):571–573. doi: 10.1016/s0140-6736(72)90360-1. [DOI] [PubMed] [Google Scholar]
  19. Payne S. V., Newell D. G., Jones D. B., Wright D. H. The Reed-Sternberg cell/lymphocyte interaction: ultrastructure and characteristics of binding. Am J Pathol. 1980 Jul;100(1):7–24. [PMC free article] [PubMed] [Google Scholar]
  20. Piovella F., Nalli G., Malamani G. D., Majolino I., Frassoni F., Sitar G. M., Ruggeri A., Dell'Orbo C., Ascari E. The ultrastructural localization of factor VIII-antigen in human platelets, megakaryocytes and endothelial cells utilizing a ferritin-labelled antibody. Br J Haematol. 1978 Jun;39(2):209–213. doi: 10.1111/j.1365-2141.1978.tb01090.x. [DOI] [PubMed] [Google Scholar]
  21. Ratnoff O. D. Antihemophilic factor (factor VIII). Ann Intern Med. 1978 Mar;88(3):403–409. doi: 10.7326/0003-4819-88-3-403. [DOI] [PubMed] [Google Scholar]
  22. Sanfilippo F., Pizzo S. V., Croker B. P. Immunohistochemical studies of cell differentiation in a juxtaglomerular tumor. Arch Pathol Lab Med. 1982 Nov;106(12):604–607. [PubMed] [Google Scholar]
  23. Wright D. H., Padley N. R., Judd M. A. Angiolymphoid hyperplasia with eosinophilia simulating lymphadenopathy. Histopathology. 1981 Mar;5(2):127–140. doi: 10.1111/j.1365-2559.1981.tb01773.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES