Abstract
The distribution of aluminium has been investigated using a new histochemical technique in transiliac bone biopsy specimens from 20 patients with aluminium related bone disease and 17 with other metabolic bone diseases. The method, which employs solochrome azurine at acid pH (acid solochrome azurine), showed a wider distribution of aluminium within cortical and trabecular bone than other histochemical techniques. The results compared well with atomic absorption spectrophotometric analysis of these biopsy specimens. Wavelength dispersive electron probe analysis of serial sections validated the procedure. The method has certain advantages over both these analytical techniques.
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- Ackrill P., Day J. P., Garstang F. M., Hodge K. C., Metcalfe P. J., Benzo Z., Hill K., Ralston A. J., Ball J., Denton J. Treatment of fracturing renal osteodystrophy by desferrioxamine. Proc Eur Dial Transplant Assoc. 1983;19:203–207. [PubMed] [Google Scholar]
- BALL J. A simple method of defining osteoid in undecalcified sections. J Clin Pathol. 1957 Aug;10(3):281–282. doi: 10.1136/jcp.10.3.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buchanan M. R., Ihle B. U., Dunn C. M. Haemodialysis related osteomalacia: a staining method to demonstrate aluminium. J Clin Pathol. 1981 Dec;34(12):1352–1354. doi: 10.1136/jcp.34.12.1352. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierides A. M., Edwards W. G., Jr, Cullum U. X., Jr, McCall J. T., Ellis H. A. Hemodialysis encephalopathy with osteomalacic fractures and muscle weakness. Kidney Int. 1980 Jul;18(1):115–124. doi: 10.1038/ki.1980.117. [DOI] [PubMed] [Google Scholar]
- Smith P. S., McClure J. Localisation of aluminium by histochemical and electron probe x-ray microanalytical techniques in bone tissue of cases of renal osteodystrophy. J Clin Pathol. 1982 Nov;35(11):1283–1293. doi: 10.1136/jcp.35.11.1283. [DOI] [PMC free article] [PubMed] [Google Scholar]