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. 1984 Jun;37(6):628–632. doi: 10.1136/jcp.37.6.628

Method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens.

J Tavares de Castro, J F San Miguel, J Soler, D Catovsky
PMCID: PMC498835  PMID: 6202720

Abstract

A method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens is described. TdT is visualised in the cell nucleus with the peroxidase-antiperoxidase (PAP) method, and the immunogold method is used in combination with monoclonal antibodies against membrane antigens. The morphology of the labelled cells is well preserved for analysis by light microscopy and the preparations obtained can be kept permanently. This method is useful for the analysis of mixed cell proliferations, particularly in leukaemias.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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