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. 1984 Aug;37(8):905–910. doi: 10.1136/jcp.37.8.905

An investigation of beta enolase as a histological marker of rhabdomyosarcoma.

J A Royds, S Variend, W R Timperley, C B Taylor
PMCID: PMC498890  PMID: 6381545

Abstract

Sections from 21 tumours diagnosed as primary or metastatic rhabdomyosarcoma were stained for alpha and beta enolase. The cases were subdivided into embryonal and alveolar subtypes (38% and 62%, respectively). Positive cytoplasmic staining for alpha enolase was seen in all but one case, and cytoplasmic staining for beta enolase was seen in some cells in 18 of the 21 cases (86% of the total, 88% of the alveolar subgroup, and 85% of the embryonal subgroup). No cells stained positively for beta enolase in the control series of neuroblastomas, fibrosarcomas, Wilms' sarcomas, and an osteosarcoma. The results show that beta enolase is a sensitive marker of muscular differentiation in rhabdomyosarcoma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bale P. M., Parsons R. E., Stevens M. M. Diagnosis and behavior of juvenile rhabdomyosarcoma. Hum Pathol. 1983 Jul;14(7):596–611. doi: 10.1016/s0046-8177(83)80203-2. [DOI] [PubMed] [Google Scholar]
  2. Bundtzen J. L., Norback D. H. The ultrastructure of poorly differentiated rhabdomyosarcomas: a case report and literature review. Hum Pathol. 1982 Apr;13(4):301–313. doi: 10.1016/s0046-8177(82)80220-7. [DOI] [PubMed] [Google Scholar]
  3. Canale V. C., Volpe R., Carbone A., Grigoletto E. Botryoid rhabdomyosarcoma of the nasopharynx. J Laryngol Otol. 1983 Jun;97(6):553–556. doi: 10.1017/s0022215100094561. [DOI] [PubMed] [Google Scholar]
  4. Corson J. M., Pinkus G. S. Intracellular myoglobin--a specific marker for skeletal muscle differentiation in soft tissue sarcomas. An immunoperoxidase study. Am J Pathol. 1981 Jun;103(3):384–389. [PMC free article] [PubMed] [Google Scholar]
  5. Dehner L. P. Soft tissue sarcomas of childhood: the differential diagnostic dilemma of the small blue cell. Natl Cancer Inst Monogr. 1981 Apr;(56):43–59. [PubMed] [Google Scholar]
  6. Fletcher L., Rider C. C., Taylor C. B., Adamson E. D., Luke B. M., Graham C. F. Enolase isoenzymes as markers of differentiation in teratocarcinoma cells and normal tissues of mouse. Dev Biol. 1978 Aug;65(2):462–475. doi: 10.1016/0012-1606(78)90041-6. [DOI] [PubMed] [Google Scholar]
  7. Fletcher L., Rider C. C., Taylor C. B. Enolase isoenzymes. III. Chromatographic and immunological characteristics of rat brain enolase. Biochim Biophys Acta. 1976 Nov 8;452(1):245–252. doi: 10.1016/0005-2744(76)90077-2. [DOI] [PubMed] [Google Scholar]
  8. Goldberg D. M., Rider C. C., Taylor C. B. Total and immunologically reacting muscle enolase in blood serum of Duchenne muscular dystrophy patients and carriers. Clin Chim Acta. 1976 Aug 16;71(1):89–93. doi: 10.1016/0009-8981(76)90279-5. [DOI] [PubMed] [Google Scholar]
  9. Herraez-Dominguez M. V., Goldberg D. M., Greaves M., Fleming J. S., Rider C. C., Taylor C. B. Immunological determination of muscle-type enolase in the serum as a diagnostic test for myocardial infarction. Clin Chim Acta. 1975 Nov 3;64(3):307–315. doi: 10.1016/0009-8981(75)90359-9. [DOI] [PubMed] [Google Scholar]
  10. Kahn H. J., Yeger H., Kassim O., Jorgensen A. O., MacLennan D. H., Baumal R., Smith C. R., Phillips M. J. Immunohistochemical and electron microscopic assessment of childhood rhabdomyosarcoma. Increased frequency of diagnosis over routine histologic methods. Cancer. 1983 May 15;51(10):1897–1903. doi: 10.1002/1097-0142(19830515)51:10<1897::aid-cncr2820511023>3.0.co;2-7. [DOI] [PubMed] [Google Scholar]
  11. Kato K., Okagawa Y., Suzuki F., Shimizu A., Mokuno K., Takahashi Y. Immunoassay of human muscle enolase subunit in serum: a novel marker antigen for muscle diseases. Clin Chim Acta. 1983 Jun 30;131(1-2):75–85. doi: 10.1016/0009-8981(83)90354-6. [DOI] [PubMed] [Google Scholar]
  12. Koh S. J., Johnson W. W. Antimyosin and antirhabdomyoblast sera: their use for the diagnosis of childhood rhabdomyosarcoma. Arch Pathol Lab Med. 1980 Mar;104(3):118–122. [PubMed] [Google Scholar]
  13. Mierau G. W., Favara B. E. Rhabdomyosarcoma in children: ultrastructural study of 31 cases. Cancer. 1980 Nov 1;46(9):2035–2040. doi: 10.1002/1097-0142(19801101)46:9<2035::aid-cncr2820460922>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  14. Miettinen M., Lehto V. P., Badley R. A., Virtanen I. Alveolar rhabdomyosarcoma. Demonstration of the muscle type of intermediate filament protein, desmin, as a diagnostic aid. Am J Pathol. 1982 Aug;108(2):246–251. [PMC free article] [PubMed] [Google Scholar]
  15. Mukai K., Rosai J., Hallaway B. E. Localization of myoglobin in normal and neoplastic human skeletal muscle cells using an immunoperoxidase method. Am J Surg Pathol. 1979 Aug;3(4):373–376. doi: 10.1097/00000478-197908000-00008. [DOI] [PubMed] [Google Scholar]
  16. Mukai K., Schollmeyer J. V., Rosai J. Immunohistochemical localization of actin: applications in surgical pathology. Am J Surg Pathol. 1981 Jan;5(1):91–97. doi: 10.1097/00000478-198101000-00013. [DOI] [PubMed] [Google Scholar]
  17. Rider C. C., Taylor C. B. Enolase isoenzymes in rat tissues. Electrophoretic, chromatographic, immunological and kinetic properties. Biochim Biophys Acta. 1974 Sep 13;365(1):285–300. doi: 10.1016/0005-2795(74)90273-6. [DOI] [PubMed] [Google Scholar]
  18. Rider C. C., Taylor C. B. Enolase isoenzymes. II. Hybridization studies, developmental and phylogenetic aspects. Biochim Biophys Acta. 1975 Sep 9;405(1):175–187. [PubMed] [Google Scholar]
  19. Royds J. A., Parsons M. A., Taylor C. B., Timperley W. R. Enolase isoenzyme distribution in the human brain and its tumours. J Pathol. 1982 May;137(1):37–49. doi: 10.1002/path.1711370105. [DOI] [PubMed] [Google Scholar]
  20. Sarnat H. B., deMello D. E., Siddiqui S. Y. Diagnostic value of histochemistry in embryonal rhabdomyosarcoma. Am J Surg Pathol. 1979 Apr;3(2):177–183. doi: 10.1097/00000478-197904000-00010. [DOI] [PubMed] [Google Scholar]
  21. Taylor C. B., Royds J. A., Parsons M. A., Timperley W. R. Diagnostic aspects of enolase isozymes. Isozymes Curr Top Biol Med Res. 1983;11:95–119. [PubMed] [Google Scholar]

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