Table 1 .
Expressed adhesin | Cell fluorescence |
Ratio | |
---|---|---|---|
Quiescent | Vortex mixed | ||
None (EV) | 142 | 161 | 1.13 |
ΔFLO11 | 987 | 994 | 1.01 |
FLO11 | 864 | 1,128 | 1.31 |
FLO1 | 889 | 1,367 | 1.55 |
Cells of each type were vortex mixed for 5 min at 2,500 rpm or left quiescent for 5 min. The cells were then stained with 500 nM ThT and assayed by flow cytometry.