Abstract
A modification of an existing selective medium for Clostridium difficile is described. Inclusion in the medium of DL nor-leucine and p-hydroxyphenylacetic acid enables identification of C difficile to be made directly from primary isolation plates by gas chromatographic detection of caproic acid and p-cresol. Plugs of agar withdrawn from the selective medium also allow the detection of cytotoxin production in vitro.
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Selected References
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- Bone E., Tamm A., Hill M. The production of urinary phenols by gut bacteria and their possible role in the causation of large bowel cancer. Am J Clin Nutr. 1976 Dec;29(12):1448–1454. doi: 10.1093/ajcn/29.12.1448. [DOI] [PubMed] [Google Scholar]
- Borriello S. P., Honour P. Concomitance of cytotoxigenic and non-cytotoxigenic Clostridium difficile in stool specimens. J Clin Microbiol. 1983 Oct;18(4):1006–1007. doi: 10.1128/jcm.18.4.1006-1007.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borriello S. P., Honour P. Simplified procedure for the routine isolation of Clostridium difficile from faeces. J Clin Pathol. 1981 Oct;34(10):1124–1127. doi: 10.1136/jcp.34.10.1124. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang T. W., Gorbach S. L. Rapid identification of Clostridium difficile by toxin detection. J Clin Microbiol. 1982 Mar;15(3):465–467. doi: 10.1128/jcm.15.3.465-467.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elsden S. R., Hilton M. G., Waller J. M. The end products of the metabolism of aromatic amino acids by Clostridia. Arch Microbiol. 1976 Apr 1;107(3):283–288. doi: 10.1007/BF00425340. [DOI] [PubMed] [Google Scholar]
- George W. L., Sutter V. L., Citron D., Finegold S. M. Selective and differential medium for isolation of Clostridium difficile. J Clin Microbiol. 1979 Feb;9(2):214–219. doi: 10.1128/jcm.9.2.214-219.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Makin T. Rapid identification of Clostridium difficile by direct detection of volatile organic acids from primary isolation media. J Clin Pathol. 1984 Jun;37(6):711–712. doi: 10.1136/jcp.37.6.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mead G. C. The amino acid-fermenting clostridia. J Gen Microbiol. 1971 Jul;67(1):47–56. doi: 10.1099/00221287-67-1-47. [DOI] [PubMed] [Google Scholar]
- Nunez-Montiel O. L., Thompson F. S., Dowell V. R., Jr Norleucine-tyrosine broth for rapid identification of Clostridium difficile by gas-liquid chromatography. J Clin Microbiol. 1983 Feb;17(2):382–385. doi: 10.1128/jcm.17.2.382-385.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips K. D., Rogers P. A. Rapid detection and presumptive identification of Clostridium difficile by p-cresol production on a selective medium. J Clin Pathol. 1981 Jun;34(6):642–644. doi: 10.1136/jcp.34.6.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poxton I. R., Byrne M. D. Demonstration of shared antigens in the genus Clostridium by an enzyme-linked immunosorbent assay. J Med Microbiol. 1984 Apr;17(2):171–176. doi: 10.1099/00222615-17-2-171. [DOI] [PubMed] [Google Scholar]
- Poxton I. R., Byrne M. D. Immunological analysis of the EDTA-soluble antigens of Clostridium difficile and related species. J Gen Microbiol. 1981 Jan;122(1):41–46. doi: 10.1099/00221287-122-1-41. [DOI] [PubMed] [Google Scholar]
- Willey S. H., Bartlett J. G. Cultures for Clostridium difficile in stools containing a cytotoxin neutralized by Clostridium sordellii antitoxin. J Clin Microbiol. 1979 Dec;10(6):880–884. doi: 10.1128/jcm.10.6.880-884.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
