Table 1.

Summary of antibodies generated for this study.

mAb	IgG Subclass	VH Family	VL Family	ka × 10−3(µM−1s−1)a	kd × 10−3(s−1)b	KD (nM)c	EC50 (µg/mL)
3C5	IgG3	Vh6	IgK V19/28	280 ± 2.0	1.1 ± 0.05	73 ± 6.0	0.9 ± 0.03
	IgG1	Vh6	IgK V19/28	16 ± 0.3	2.4 ± 0.14	1460 ± 81	18 ± 1.7
	IgG2b	Vh6	IgK V19/28	27 ± 0.3	2.4 ± 0.09	790 ± 120	12 ± 0.8
	IgG2a	Vh6	IgK V19/28	43 ± 0.7	4.1 ± 0.11	550 ± 120	12 ± 1.0
	F(ab')2d	—	—	38 ± 2.0	5.9 ± 0.29	2100 ± 610	383 ± 130
2A5	IgG1	Vh6	IgK V21	1800 ± 120	24 ± 1.6	43 ± 6.0	0.37 ± 0.02
	IgG2b	Vh6	IgK V21	1100 ± 50	12 ± 0.51	36 ± 4.3	0.36 ± 0.03
	IgG2a	Vh6	IgK V21	720 ± 30	9.3 ± 0.35	58 ± 8.1	0.76 ± 0.08

^aAssociation rate constant

^bDissociation rate constant

^cDissociation constant

^dIgG₃ F(ab')₂