Abstract
A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bartlett A., Dormandy K. M., Hawkey C. M., Stableforth P., Voller A. Factor-VIII-related antigen: measurement by enzyme immunoassay. Br Med J. 1976 Apr 24;1(6016):994–996. doi: 10.1136/bmj.1.6016.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fishman D. J., Jones P. K., Menitove J. E., Ratnoff O. D., Everson B. Detection of the carrier state for classic hemophilia using an enzyme-linked immunosorbent assay (ELISA). Blood. 1982 Jun;59(6):1163–1168. [PubMed] [Google Scholar]
- Peake I. R., Bloom A. L. The use of an immunoradiometric assay for factor VIII related antigen in the study of atypical Von Willebrand's disease. Thromb Res. 1977 Jan;10(1):27–32. doi: 10.1016/0049-3848(77)90078-0. [DOI] [PubMed] [Google Scholar]
- Richardson S. G., Matthews K. B., Stuart J., Geddes A. M., Wilcox R. M. Serial changes in coagulation and viscosity during sickle-cell crisis. Br J Haematol. 1979 Jan;41(1):95–103. doi: 10.1111/j.1365-2141.1979.tb03685.x. [DOI] [PubMed] [Google Scholar]
- Short P. E., Williams C. E., Picken A. M., Hill F. G. Factor VIII related antigen: an improved enzyme immunoassay. Med Lab Sci. 1982 Oct;39(4):351–355. [PubMed] [Google Scholar]
- Yolken R. H., Leister F. J. Investigation of enzyme immunoassay time courses: development of rapid assay systems. J Clin Microbiol. 1981 Apr;13(4):738–741. doi: 10.1128/jcm.13.4.738-741.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yorde L. D., Hussey C. V., Yorde D. E., Sasse E. A. Competitive enzyme-linked immunoassay for Factor VIII antigen. Clin Chem. 1979 Nov;25(11):1924–1927. [PubMed] [Google Scholar]
- Zimmerman T. S., Ratnoff O. D., Littell A. S. Detection of carriers of classic hemophilia using an immunologic assay for antihemophilic factor (factor 8(). J Clin Invest. 1971 Jan;50(1):255–258. doi: 10.1172/JCI106481. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Dieijen G., Tans G., Rosing J., Hemker H. C. The role of phospholipid and factor VIIIa in the activation of bovine factor X. J Biol Chem. 1981 Apr 10;256(7):3433–3442. [PubMed] [Google Scholar]