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. 2016 Mar 1;7(15):20410–20424. doi: 10.18632/oncotarget.7804

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Copyright: © 2016 Juengel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Magnet bead separation of Caki-1 and HUVEC from the co-culture by epithelial CD326. Black line: isotype control, green line: unstimulated cells, red line: TNF-alpha (24h, 500 U/ml) stimulated cells. One representative of three separate experiments is shown. B. Western Blot Analysis. Total protein content of ICAM-1, VCAM-1, E-selectin and CD144 (endothelial control) in Caki-1 and HUVEC cells separated by magnet beads. HUVEC + Caki = HUVEC separated from Caki-1 co-culture. Caki-1 and HUVEC cells, cultivated separately, served as controls. n.d. = not detectable. C. Pixel density of protein bands shown in B.