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. 2016 Mar 1;7(15):20549–20560. doi: 10.18632/oncotarget.7823

Figure 3. High salt and Hp lysate can up regulate SGK1 and Lnc-SGk1 expression.

Figure 3

A-D. T cells were treated with either different concentration of NaCl or Hp and E. coli lysate and then the expression of both SGK1 and Lnc-SGK1 was detected by real-time PCR. E-F. T cells were modified with specific siRNA to SGK1 and JunB, and next the T cells were treated with 40nM NaCl and Hp lysate. G. T cells was transected with Lnc-SGK1 and treated with 40nM NaCl and Hp lysate the expression of SGK1 and phosphorated JunB was detected by western-blot. H. Luciferase reporter analysis was performed using promoter 2Kb ahead of start code; mutagenesis was performed based on different JunB binding sites. Each experiment was performed three times separately, P < 0.05 was considered as significance.