Figure 4. Lnc-SGK1 can promote Th2 and Th17 differentiation through SGK1/JunB signaling.

A. Polarization stimulations were carried out to Th1 Th2 and Th17 respectively, T cells was transected indicated in the figure, the percentage of each cells was determined by flow cytometry. B. Transcriptional factor of different type of T help cell were determined by western-blot in polarization stimulated T cells modified indicated in the figure. T cells modified indicating in the figure and co-culture with either NaCl and Hp lysate, the percentage of Th1, Th2 and Th17 were respectively determined by flow cytometry and presented in figure C, D and E. Moreover, the transcriptional factors of T helper cell were detected by western-blot and further presented in F. Each experiment was performed three times separately, P < 0.05 was considered as significance.