Skip to main content
. 2016 Mar 7;7(15):20597–20611. doi: 10.18632/oncotarget.7972

Figure 2. MEK1 signaling activity is required for the maintenance of liver CSC self-renewal.

Figure 2

(A) Huh7-NanogPos cells were co-cultured with various concentrations of U0126 (0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM, 20 μM) in sphere-forming conditions for 7 days, counted at the same magnification. (B) Huh7-NanogPos cells were treated with different concentrations of U0126 (0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM, 20 μM) and grown for 14 days. Cells were stained with crystal violet and counted. (C) Western blot analysis of stemness protein expression in Huh7- and PLC/PRF/5-NanogPos cells, which co-cultured with various concentrations of U0126 (0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM, 20 μM) for 48 hours. (D) Huh7-NanogPos cells were treated with 5 μM U0126 for 14 days, while the negative control treated with DMSO for 14 days. Then we subcutaneous injected 1 × 102, 1 × 103, 1 × 104 cells into NOD-SCID mice. After 30 days, we harvested and counted the tumors. Extreme Limiting Dilution Analysis was acquired from http://bioinf.wehi.edu.au/software/elda/.